L020000

TCA Deblock

• Code UNSPSC : 41116004
• Nomenclature NACRES : NA.21

Propriétés

• Type: for DNA synthesis; for DNA synthesis
• Niveau de qualité: 200
• Gamme de produits: Proligo Reagents
• Forme: liquid
• Conditions de stockage: dry at room temperature
• Concentration: 28-32 g/L (TCA content, titration)
• Technique(s): DNA synthesis: suitable
• Impuretés: ≤200 ppm water content (Karl Fischer)
• Couleur: clear; clear
• Point d'ébullition: 39.8—40.0 °C/1,013 hPa
• Température de stockage: room temp

Description

Notes préparatoires

Trichloroacetic acid / Dichloromethane 3/100 (v/v)

Autres remarques

Deblock solution containing trichloroacetic acid (TCA) in dichloromethane removes the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

Informations sur la sécurité

• Pictogrammes: GHS08,GHS05,GHS07,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H315,H318,H335,H336,H351,H411
• Conseils de prudence: P202 - P273 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Classification des risques: Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Central nervous system, Respiratory system
• Code de la classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): 212.0 °F - closed cup
• Point d'éclair (°C): 100 °C - closed cup