860891

Bromocresol Purple sodium salt

indicator grade, Dye content 90 %

• Synonyme(s) : 5′,5″-Dibromo-o-cresolsulfonphthalein sodium salt
• Formule empirique (notation de Hill): C₂₁H₁₅Br₂NaO₅S
• Numéro CAS: 62625-30-3
• Poids moléculaire : 562.20
• Numéro CE : 263-655-3
• Numéro MDL: MFCD00148896
• Code UNSPSC : 12171500
• ID de substance PubChem : 24888522
• Nomenclature NACRES : NA.47

Propriétés

• Qualité: indicator grade
• Niveau de qualité: 200
• Forme: powder
• Composition: Dye content, 90%
• Technique(s): titration: suitable
• Couleur: brown to very dark brown, to Very Dark Red
• visual transition interval: 5.2-6.8, yellow to purple
• Pf: 255 °C (dec.) (lit.)
• λmax: 379 nm; 585 nm (2nd)
• ε (coefficient d'extinction): ≥47000 at 587-591 nm in 0.1 M NaOH; ≥7000 at 375-381 nm in 0.1 M NaOH
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: [Na+].Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c([O-])c(Br)c4
• InChI: 1S/C21H16Br2O5S.Na/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21;/h3-10,24-25H,1-2H3;/q;+1/p-1
• Clé InChI: WNOIORUCCYNQPE-UHFFFAOYSA-M

Description

Description générale

Bromocresol Purple, also known as dibromo-o-cresolsulfonphthalein, is an acidic dye and belongs to the phthalein and sulphonphthalein dyes group. The dye color changes from yellow to violet at pH 5.2-6.8. The monosodium salt of the dye is 8% soluble in water and 2% in ethanol. Bromocresol Purple, under alkaline conditions, forms a hydrophilic, moderately sized dianion that is purple.

Application

Bromocresol Purple is widely used as a vital stain to trace fluid diffusion in the lymphatic system. In addition, the dye is suitable to differentiate dead or live yeast cells. As a pH indicator, Bromocresol Purple is used in microbial culture media, both broth, and agar. In analytical experiments, e.g., to stain carbonic anhydrase isoenzyme on cellulose acetate electrophoresis membranes, bromocresol purple is used.

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H315,H319,H335
• Conseils de prudence: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves