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219215

Sigma-Aldrich

Leucocrystal Violet

Synonyme(s) :

4,4′,4′′-Methylidynetris(N,N-dimethylaniline), Leuco Crystal Violet

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About This Item

Formule linéaire :
CH[C6H4N(CH3)2]3
Numéro CAS:
603-48-5
Poids moléculaire :
373.53
Numéro CE :
210-043-9
Numéro MDL:
MFCD00008314
Code UNSPSC :
12171500
ID de substance PubChem :
24853114
Nomenclature NACRES :
NA.47

Forme

powder

Technique(s)

microbe id | staining: suitable

Pf

175-177 °C (lit.)

λmax

260 nm

ε (coefficient d'extinction)

≥700 at 257-263 nm

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

Chaîne SMILES 

CN(C)c1ccc(cc1)C(c2ccc(cc2)N(C)C)c3ccc(cc3)N(C)C

InChI

1S/C25H31N3/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6/h7-18,25H,1-6H3

Clé InChI

OAZWDJGLIYNYMU-UHFFFAOYSA-N

Application

Leucocrystal Violet has been used to identify histamine-producing lactobacilli in cheese.

Actions biochimiques/physiologiques

Leucocrystal Violet (LCV) is a cationic triarylmethane dye. LCV is a reduced form of gentian violet (crystal violet) and thus, it is colorless. It has an affinity for both cellulosic and proteinaceous materials. LCV and hydrogen peroxide react with the hemoglobin in blood and turn the blood impression to purple/violet color. Therefore, the dye is widely used to stain blood residue on both porous and non-porous materials.

Attention

Photo-ionization can cause color variations

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Environment
GHS09

Mention d'avertissement

Warning

Mentions de danger

H410

Conseils de prudence

P273 - P391 - P501

Classification des risques

Aquatic Acute 1 - Aquatic Chronic 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Gloves

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Certificats d'analyse (COA)

Lot/Batch Number
0000236216
MKBW1177V
MKBR8820V
MKBR8440V
S41106

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

J E Roybal et al.
Journal of chromatography, 467(1), 259-266 (1989-04-21)
High-performance liquid chromatographic conditions are reported for the electrochemical detection (ED) of Gentian Violet, its demethylated metabolites, Leucogentian Violet and Methylene Blue. Gentian Violet, its demethylated metabolites and Leucogentian Violet were separated within 14 min on a cyano column eluted
R T Wilson et al.
Journal of AOAC International, 77(5), 1137-1142 (1994-09-01)
A gas chromatographic/mass spectrometric (GC/MS) procedure for confirming the identity of leucogentian violet (LGV) in chicken fat was developed for regulatory application. The unused portion of the extract remaining from a determinative procedure was back-extracted into an organic phase, concentrated
J J McDonald et al.
Drug metabolism and disposition: the biological fate of chemicals, 12(3), 330-336 (1984-05-01)
Cellular suspensions of microflora isolated from human feces and from intestinal contents of rat and chicken transformed the triphenylmethane dye gentian violet under anaerobic conditions. Pure cultures of nine genera of strict and facultative anaerobes were also active. All pure
Xueli Wu et al.
Journal of chromatography. A, 1172(2), 121-126 (2007-10-24)
A method has been developed for the simultaneous determination of malachite green, gentian violet and their leuco-metabolites in various aquatic products using isotope dilution liquid chromatography-linear ion trap mass spectrometry without post-column oxidation. Sample was extracted with McIlvaine buffer and
P G Schermerhorn et al.
Journal of AOAC International, 77(6), 1454-1460 (1994-11-01)
A laboratory trial was completed for a liquid chromatographic method that can quantitate leucogentian violet (LGV) in chicken fat at 10 ppb. With this method, LGV is isolated from the fat matrix by a series of liquid-liquid extractions. This trial
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