M217

Monoclonal Anti-Nicotinic Acetylcholine Receptor (α₁, α₃, α₅ Subunits) antibody produced in rat

clone mAb 35, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-ACHR
• MDL number: MFCD00677279
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rat
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: mAb 35, monoclonal
• form: buffered aqueous solution
• species reactivity: human, chicken, eel
• should not react with: Xenopus
• technique(s): immunohistochemistry (frozen sections): 1:3,000
• isotype: IgG1
• UniProt accession no.: CHRNA1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CHRNA1(1134) , CHRNA3(1136) , CHRNA5(1138)

Description

Specificity

Binds to the main immunogenic region on the extracellular surface of the α₁ subunit of muscle AChR′s and to homologous regions on α₃ and α₅ subunits of neuronal AChR′s. The epitope is located on the extracellular surface of the α₁ subunit of the muscle nicotinic acetylcholine receptor. The antibody binds to denatured chick α₅ AChR, but does not bind well to denatured α₃, α₄, α₇, α_8, and β₂. It may also bind β₃ due to homology of main immunogenic region. May be used to localize and detect the α₁, α₃ and α₅ subunits of the nicotinic acetylcholine receptor (AChR).

Immunogen

nicotinic acetylcholine receptor from the electric eel Electrophorus.

Physical form

Solution in 20 mM Na₃PO₄, pH 7.2, containing 150 mM NaCl and 0.05 % sodium azide.

Legal Information

Sold with permission of the Salk Institute

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves