fR2

98031102, human breast, Epithelial, cuboidal

• UNSPSC Code: 41106514

Properties

• product name: fR2, 98031102
• biological source: human breast
• growth mode: Adherent
• karyotype: Aneuploid
• morphology: Epithelial, cuboidal
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

For complete product information, please see fR2

Cell Line Origin

Human breast epithelial, SV40 transformed

Cell Line Description

The SV40 transformed breast epithelial cell line fR2 was obtained in 1982 by infecting suspensions of primary milk cultures with wild-type SV40. The cells have been shown to express Muc-1, also known as HMFG-1 antigen, which is a mucin-like component of human milk fat globule membranes. They are positive for keratin 8 and 18, but negative for keratins 4, 6, 7, 10, 13, 14, 16, 17 and 19. Although fR2 is morphologically different from fR5 (Sigma Catalogue no. 98031103), they have probably been derived from a common precursor cell since the karyotypes are similar. Karyotype analysis revealed hypotetraploidy and several rearrangements involving chromosome 1 and 11 which are frequently found in breast carcinomas and lines derived from metastatic pleural effusions. fR2 cells display anchorage independent growth in soft agar and are positive for SV40 T-antigen. Early passages were non-tumorigenic in nude mice.

Application

Study of human mammary epithelial cells

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 12,13
D13S317: 12
D16S539: 11,13
D5S818: 12,13
D7S820: 8,12
THO1: 9.3
TPOX: 8
vWA: 20

Culture Medium

RPMI 1640 + 2mM Glutamine + 10μg/ml insulin + 5μg/ml hydrocortisone + 10% FBS

Subculture Routine

Split sub-confluent cultures (70-80%) 1:10 i.e. seeding 1 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

Other Notes

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