IM 9

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86051302, human bone marrow, Lymphoblast

• UNSPSC Code: 41106514

Properties

• biological source: human bone marrow
• packaging: tube of 5 μg 86051302-DNA-5UG; pkg of vial of cells 86051302-1VL
• growth mode: Suspension
• karyotype: 2n = 46
• morphology: Lymphoblast
• products: Immunoglobulin G (IgG)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human B lymphocyte

Cell Line Description

Derived from bone marrow removed from a female patient with multiple myeloma. The cells synthesise IgG at a rate of 2.4ug/1,000,000 cells/day and have receptors for insulin, calcitonin and human growth hormone. They also exhibit surface markers and receptor sites characteristic of B lymphocytes.

Application

Biosynthetic and surface marker studies

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 2-4x100,000 cells/ml; 5% CO₂; 37°C. Maximum cell density 1,000,000 cells/ml.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.