TLC Analysis Of Vitamin C Derivates From Cosmetic Formulation on HPTLC Silica gel 60 F254s
Matériaux
À utiliser avec
Réf. du produit
Description
Tarif
Cuve de développement de plaques CCM Aldrich® - rectangulaires
L × H × W 27.0 cm × 26.5 cm × 7.0 cmCuve de développement de plaques CCM Aldrich® - rectangulaires
L × H × W 7.5 cm × 15.5 cm × 8.0 cmSeringues TLC Hamilton®
1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),coated tip
Seringues TLC Hamilton®
1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
Seringues TLC Hamilton®
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
Cuve de développement de plaques CCM Aldrich® - rectangulaires
L × H × W 17.5 cm × 16.0 cm × 8.2 cmSeringues TLC Hamilton®
1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
CONDITIONS
sample preparation
1 g cosmetic formulation (1% and 2% active) was stirred in 10 ml THF for 15 min and filtered over a 0.45 μm syringe filter. Application by CAMAG ATS4 6 mm bandwise.
plate
1.15161 HPTLC Silica gel 60 RP-18 F 254s MS-grade 20cm x 10 cm (1.151161.0001)
eluent
[A] methanol; [B] acetonitrile; (9:1, A:B)
migration distance
6 cm
chamber
normal chamber without chamber saturation
sample
0.150 μL
detector
UV, 254 nm (Identification by coupling the TLC – MS Interface (CAMAG) with the Advion expression) (CMS mass spectrometer (+)-ESI mode. Eluent: Acetonitrile / Water 95:5 + 0.1% TFA.)