Accéder au contenu
Merck

Fluorescent carbon nanodots for sensitive and selective detection of tannic acid in wines.

Talanta (2014-12-06)
Gaber Hashem Gaber Ahmed, Rosana Badía Laíño, Josefa Angela García Calzón, Marta Elena Díaz García
RÉSUMÉ

Herein we describe an easy one step synthesis of carbon nanodots (C-dots) by thermal carbonization of 6-bromohexylboronic acid using two different amine compounds, polyethyleneglycol bis(3-aminopropyl (PEGA) and 1,2-aminopropane (DPA), at 180 °C in atmospheric oxygen. The as-synthesized C-dots were characterized by FTIR, HRTEM, NMR and fluorescence. The C-dots prepared using PEGA showed a strong emission at 440 nm with excitation at 362 nm. These C-dots exhibited analytical potential as sensing probes for tannic acid (TA) determination. pH effect, interferences, and analytical performance of the method were investigated. The method was found effective in the linear concentration range from 0.1 to 10 mg L(-1) TA achieving a limit of detection equal 0.018 mg L(-1) TA. The applicability of the method was demonstrated by direct measurements of TA in red and white wine samples. Validation of the method was achieved by spiking the wine samples with different standard TA concentrations obtaining recoveries in the range (90-112.5%). A probable mechanism by which TA quenched the C-dots fluorescence was proposed.

MATÉRIAUX
Référence du produit
Marque
Description du produit

Sigma-Aldrich
Chlorure de potassium, ACS reagent, 99.0-100.5%
Sigma-Aldrich
Hydroxyde de sodium, ACS reagent, ≥97.0%, pellets
Sigma-Aldrich
Phosphate de sodium dibasic, ACS reagent, ≥99.0%
Sigma-Aldrich
Hydroxyde de sodium, reagent grade, ≥98%, pellets (anhydrous)
Sigma-Aldrich
Hydroxyde de sodium solution, 50% in H2O
Sigma-Aldrich
Saccharose, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
Saccharose, ≥99.5% (GC)
Sigma-Aldrich
Sodium fluoride, ACS reagent, ≥99%
Sigma-Aldrich
Hydroxyde de sodium solution, BioUltra, for molecular biology, 10 M in H2O
Sigma-Aldrich
Phosphate de sodium dibasic, puriss. p.a., ACS reagent, anhydrous, ≥99.0% (T)
Sigma-Aldrich
Saccharose, ≥99.5% (GC), BioXtra
Sigma-Aldrich
Sulfite de sodium, ACS reagent, ≥98.0%
Sigma-Aldrich
L-acide ascorbique, powder, suitable for cell culture, γ-irradiated
Sigma-Aldrich
L-acide ascorbique, BioXtra, ≥99.0%, crystalline
Sigma-Aldrich
D-(−)-fructose, ≥99% (HPLC)
Sigma-Aldrich
Chlorure de calcium solution, BioUltra, for molecular biology, ~1 M in H2O
Sigma-Aldrich
Saccharose, BioUltra, for molecular biology, ≥99.5% (HPLC)
Sigma-Aldrich
Chlorure de potassium, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, E508, 99-100.5% (AT), ≤0.0001% Al
Sigma-Aldrich
Hydroxyde de sodium, BioXtra, ≥98% (acidimetric), pellets (anhydrous)
Sigma-Aldrich
Hydroxyde de sodium solution, 1.0 N, BioReagent, suitable for cell culture
Sigma-Aldrich
Chlorure de potassium, for molecular biology, ≥99.0%
Sigma-Aldrich
Hydroxyde de sodium, puriss., meets analytical specification of Ph. Eur., BP, NF, E524, 98-100.5%, pellets
Sigma-Aldrich
Acide citrique, meets analytical specification of Ph. Eur., BP, USP, E330, anhydrous, 99.5-100.5% (based on anhydrous substance)
Sigma-Aldrich
Sulfite de sodium, ≥98%
Sigma-Aldrich
Hydroxyde de sodium, reagent grade, 97%, powder
Sigma-Aldrich
Chlorure de potassium, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99.0%
Sigma-Aldrich
Hydroxyde de sodium, pellets, semiconductor grade, 99.99% trace metals basis
Sigma-Aldrich
Calcium chloride, anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%
USP
Saccharose, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Chlorure de potassium, ReagentPlus®, ≥99.0%