- Identification of genes potentially involved in supporting hematopoietic stem cell activity of stromal cell line MC3T3-G2/PA6.
Identification of genes potentially involved in supporting hematopoietic stem cell activity of stromal cell line MC3T3-G2/PA6.
International journal of hematology (2008-03-05)
Natsumi Shimizu, Shinichi Noda, Kazufumi Katayama, Hitoshi Ichikawa, Hiroaki Kodama, Hiroyuki Miyoshi
PMID18317883
RÉSUMÉ
Although coculture of hematopoietic stem cells (HSCs) with stromal cells is a useful system to study hematopoiesis in the niche, little is known regarding the precise cellular and molecular mechanisms of maintaining HSCs through cell-cell interactions. The murine preadipose stromal cell line MC3T3-G2/PA6 (PA6) has been demonstrated to support HSCs in vitro. In this study, microarray analysis was performed on PA6 cells and HSC-nonsupporting PA6 subclone cells to identify genes responsible for supporting HSC activity. Comparison of gene expression profiles revealed that only 144 genes were down-regulated by more than twofold in PA6 subclone cells. Of these down-regulated genes, we selected 11 candidate genes and evaluated for the maintenance of HSC function by overexpressing these genes in PA6 subclone cells. One unknown gene, 1110007F12Rik (also named as Tmem140), which is predicted to encode an integral membrane protein, demonstrated a partial restoration of the defect in HSC-supporting activity.
MATÉRIAUX
Référence du produit
Marque
Description du produit
Sigma-Aldrich
Sérum de veau fœtal, USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma
Sigma-Aldrich
Sérum de veau fœtal, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma
Sigma-Aldrich
Milieu essentiel minimum d′Eagle, With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Sérum de veau fœtal, Heat Inactivated, non-USA origin, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Milieu essentiel minimum d′Eagle, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Sérum de veau fœtal, non-USA origin, from USDA approved countries, Heat Inactivated, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Milieu essentiel minimum d′Eagle, With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Milieu essentiel minimum d′Eagle, Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Sérum de veau fœtal, USA origin, Charcoal Stripped, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Milieu essentiel minimum d′Eagle, Alpha Modification, with ribonucleosides, deoxyribonucleosides and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Sérum de veau fœtal, USA origin, γ-irradiated by SER-TAIN process, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Milieu essentiel minimum d′Eagle, With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture
Sigma-Aldrich
Sérum de veau fœtal, Australia origin, USDA approved, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Sérum de veau fœtal, USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Sérum de veau fœtal, USA origin, Dialyzed by ultrafiltration against 0.15 M NaCl, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Milieu essentiel minimum d′Eagle, with Earle′s salts and non-essential amino acids, without L-glutamine, phenol red and sodium bicarbonate, Modified, powder, suitable for cell culture
Sigma-Aldrich
Sérum de veau fœtal, Australia origin, γ-irradiated, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Milieu essentiel minimum d′Eagle, With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Sigma-Aldrich
Minimum Essential Medium Eagle, With Earle′s salts, stable glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Milieu essentiel minimum d′Eagle, Alpha Modification, with L-glutamine, ribonucleosides and deoxyribonucleosides, without sodium bicarbonate, powder, suitable for cell culture
Sigma-Aldrich
Sérum de veau fœtal, Australia origin, suitable for, USDA approved, sterile-filtered, suitable for cell culture, suitable for hybridoma
Sigma-Aldrich
Milieu essentiel minimum d′Eagle, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Milieu essentiel minimum d′Eagle, HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Milieu essentiel minimum d′Eagle, With stable glutamine and sodium bicarbonate, without ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Milieu essentiel minimum d′Eagle, Alpha Modification, with L-glutamine and sodium pyruvate, without ribonucleosides, deoxyribonucleosides and sodium bicarbonate, powder, suitable for cell culture
Sigma-Aldrich
Milieu essentiel minimum d′Eagle, With stable glutamine and sodium bicarbonate, with ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Milieu essentiel minimum d′Eagle, With Hanks′ salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Milieu essentiel minimum d′Eagle, Modified, with Earle′s salts and reduced NaHCO3. without L-glutamine, liquid, sterile-filtered, suitable for cell culture