M0275

Milieu essentiel minimum d′Eagle

With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, sterile liquid, suitable for cell culture, 10x

• Synonyme(s) : MEM
• Code UNSPSC : 12352207
• Nomenclature NACRES : NA.71

Propriétés

• product name: Milieu essentiel minimum d′Eagle, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
• Stérilité: sterile-filtered
• Forme: liquid
• Concentration: 10 ×
• Technique(s): cell culture | mammalian: suitable
• Impuretés: endotoxin, tested
• Composants: phenol red: yes; HEPES: no; Earle’s salts (5% CO₂): yes; L-glutamine: no; sodium pyruvate: no; NaHCO₃: no
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Application

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera. The L-glutamine and sodium bicarbonate are added to the medium after dilution to 1x and pH adjustment.

Minimum Essential Medium Eagle has been used for the formulation of collagen methacrylamide (hydrogel). It has also been used to maintain articular cartilage of bovine knee joints, which was later used for MRI (magnetic resonance imaging) study and histological analysis.

Reconstitution

Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable