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M8042

Sigma-Aldrich

Milieu essentiel minimum d′Eagle

Alpha Modification, with nucleosides and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonyme(s) :

MEM

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500 ML
69,60 €
6 X 500 ML
339,00 €
24 X 500 ML
1 120,00 €

69,60 €

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Devis pour commande en gros
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Sélectionner une taille de conditionnement

Changer de vue
500 ML
69,60 €
6 X 500 ML
339,00 €
24 X 500 ML
1 120,00 €

About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

69,60 €

En stockDétails

Devis pour commande en gros

Nom du produit

Milieu essentiel minimum d′Eagle, Alpha Modification, with ribonucleosides, deoxyribonucleosides and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Stérilité

sterile-filtered

Forme

liquid

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Composants

Hanks’ salts (2% CO2): no
glucose: yes
sodium pyruvate: yes
phenol red: yes
HEPES: no
L-glutamine: no
stable glutamine: no
Earle’s salts (5% CO2): yes
NaHCO3: yes

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Description générale

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.[1]This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Often used in cytogenetic analysis when supplemented with fetal bovine serum (FBS).

Application

Minimum Essential Medium Eagle has been used for the isolation of mesenchymal stem cell, adipose-derived stem cell,[2] and osteoblast.[3]
Minimum Essential Medium Eagle is used for primary osteoblast cultures[4] and for isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.[5]

Actions biochimiques/physiologiques

MEM usually autoclaved with 0.5% bactopeptone at 15 psi for 20 min, cooled and then neutralized with NaHCO3, consistently supported good cell growth. A similar result was obtained when the MEM and bactopeptone were autoclaved separately. The cells grow, initially, as a monolayer and subsequently become a stationary suspension.[6]

Reconstitution

Supplement with 0.292 g/L L-glutamine

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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
RNBL9393
RNBL0710
RNBK3712
RNBK2051
RNBJ4241

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Isolation of adult stem cells and their differentiation to Schwann cells
Progenitor Cells, 47-57 (2012)
L Keay
Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01)
The growth of L-60TM cells (a suspension culture adapted L-cell) on media composed of MEM (minimum essential medium (Eagle)) and bactopeptone autoclaved together or separately under a variety of conditions has veen determined. It has been found that MEM autoclaved
A Maharaj et al.
The Journal of steroid biochemistry and molecular biology, 202, 105730-105730 (2020-07-20)
Deficiency in Sphingosine-1-phosphate lyase (S1P lyase) is associated with a multi-systemic disorder incorporating primary adrenal insufficiency (PAI), steroid resistant nephrotic syndrome and neurological dysfunction. Accumulation of sphingolipid intermediates, as seen with loss of function mutations in SGPL1, has been implicated
A previously functional tetracycline-regulated transactivator fails to target gene expression to the bone
Schmidt E and Eriksson M
BMC Research Notes, 4(1), 282-282 (2011)
Jennifer J P Collins et al.
Stem cells and development, 27(16), 1109-1124 (2018-06-30)
Bronchopulmonary dysplasia (BPD), the most common complication of extreme preterm birth, can be caused by oxygen-related lung injury and is characterized by impaired alveolar and vascular development. Mesenchymal stromal cells (MSCs) have lung protective effects. Conversely, BPD is associated with
Afficher tous les articles scientifiques apparentés

Articles

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion

PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.

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