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54809

Sigma-Aldrich

Mono P® 5/50 GL

10 μm particle size, L × I.D. 5 cm × 5 mm

Synonyme(s) :

Colonnes d’échange d’ions Amersham Biosciences

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About This Item

Code UNSPSC :
23151817
Nomenclature NACRES :
NA.56

L × D.I.

5 cm × 5 mm

Matrice

MonoBeads

Groupe de la matrice active

amphoteric

Taille des particules

10 μm

Compatibilité

mode of use ion exchange

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Description générale

Mono columns are highly efficient, pH-stable columns designed for high performance ion exchange separations of proteins, peptides, and polynucleotides. The unique properties of these columns are based on MonoBeads support, a beaded hydrophilic material with the narrowest particle size distribution of any chromatographic support. This monodispersity permits high flow rates at relatively low backpressures.

Application

Mono columns are used in peptide mapping and monoclonal antibody purification. Mono P columns have been used to purify homogenous forms of fungal peroxygenase and to investigate separation of DNA restriction fragments.

Informations légales

Mono P is a registered trademark of Cytiva

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René Ullrich et al.
Biotechnology journal, 4(11), 1619-1626 (2009-07-09)
Extracellular peroxygenase from the agaric fungus Agrocybe aegerita is a versatile biocatalyst that oxygenates various substrates by means of hydrogen peroxide. The enzyme is routinely produced in suspensions of soybean meal and has until now been purified by several steps
E Westman et al.
Analytical biochemistry, 166(1), 158-171 (1987-10-01)
Separation of DNA restriction fragments by FPLC ion-exchange chromatography on Mono Q and Mono P columns was investigated. The columns were found to be particularly suitable for the separation of fragments up to 500-600 bp long. Larger fragments can also

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