GE17-5181-01
SOURCE™ 15Q 4.6/100 PE
Synonyme(s) :
SOURCE 15Q Column
About This Item
Produits recommandés
ligand
quaternary ammonium
Matériaux
polypropylene column
Description
Ion Exchanger Type (value)
Conditionnement
pkg of 1 ea
Paramètres
580 psi
Taille du lit
4.6 mm × 100 mm
Volume du lit
1.7 mL
D.I. de la colonne
4.6 mm
Matrice
polymeric
polystyrene/divinylbenzene
Groupe de la matrice active
ammonium ion, quaternary phase
Taille moy. des particules
15 μm
cleaning
1-14
working range
2-12
Adéquation
suitable for bioprocess medium
Technique de séparation
anion exchange
Catégories apparentées
Description générale
The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE™ 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.
Caractéristiques et avantages
- SOURCE™ 15Q media is highly suitable for fast, high-resolution purifications and easy scale-up
- Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes
- Improved capacity compared to MonoBeads but with a slightly lower resolution.
Stockage et stabilité
Remarque sur l'analyse
Autres remarques
Informations légales
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Code de la classe de stockage
3 - Flammable liquids
Certificats d'analyse (COA)
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This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
Protocoles
This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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