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TOX6

Sigma-Aldrich

In Vitro Toxicology Assay Kit, Sulforhodamine B based

Synonyme(s) :

biomass viability assay, sulforhodamine B, total biomass assay

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.84

Forme

liquid

Utilisation

 kit sufficient for 1,000 tests

Conditionnement

pkg of 1 kit

Conditions de stockage

dry at room temperature

λmax

565 nm

Application(s)

cell analysis
detection

Méthode de détection

colorimetric

Température de stockage

room temp

Description générale

The Sulforhodamine B method is simple, accurate, and provides consistent results. This assay system is a means of measuring total biomass by staining cellular proteins with sulforhodamine B.

Application

In Vitro Toxicology Assay Kit, Sulforhodamine B based has been used for spectrophotometric measurement of biomass (viable and non-viable cells) by total protein. It has also been used to study the effect of 17-allylamino-17-demethoxygeldanamycin (17-AAG) on proliferation of the five uveal melanoma cell lines. Absorbance of dye is measured at a wavelength of 565nm.

Actions biochimiques/physiologiques

Dye binds to cellular protein and is then solubilized in base.

Pictogrammes

FlameCorrosionEnvironment
GHS02,GHS05,GHS09

Mention d'avertissement

Danger

Mentions de danger

H226,H314,H410

Classification des risques

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1A

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

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P Skehan et al.
Journal of the National Cancer Institute, 82(13), 1107-1112 (1990-07-04)
We have developed a rapid, sensitive, and inexpensive method for measuring the cellular protein content of adherent and suspension cultures in 96-well microtiter plates. The method is suitable for ordinary laboratory purposes and for very large-scale applications, such as the
Marike Gabrielson et al.
PloS one, 9(9), e107109-e107109 (2014-09-23)
Epithelial ovarian carcinoma (EOC), the major cause of gynaecological cancer death, is a heterogeneous disease classified into five subtypes. Each subtype has distinct clinical characteristics and is associated with different genetic risk factors and molecular events, but all are treated
Differential sensitivities to lactate transport inhibitors of breast cancer cell lines
Santos F, eta l.
Endocrine-related cancer, 21(1), 1-40 (2014)
Evaluation of colorimetric protein and biomass stains for assaying drug effects upon human tumor cell lines.
Skehan, P., et al.
Proceedings of the American Association For Cancer Research, 30, 2436-2436 (1989)
Eduardo C A Silva et al.
Oncotarget, 9(29), 20386-20398 (2018-05-15)
Metabolic reprogramming is one of the hallmarks of cancer. The hyperglycolytic phenotype is often associated with the overexpression of metabolism-associated proteins, such as monocarboxylate transporters (MCTs). MCTs are little explored in germ cell tumors (GCTs), thus, the opportunity to understand
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