TOX4
In Vitro Toxicology Assay Kit, Neutral Red based
Synonyme(s) :
NR uptake assay, Neutral red assay
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About This Item
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.84
Produits recommandés
Forme
liquid
Utilisation
kit sufficient for 1,000 tests
Conditionnement
pkg of 1 kit
Conditions de stockage
dry at room temperature
λmax
540 nm
Application(s)
cell analysis
detection
Méthode de détection
colorimetric
Température de stockage
2-8°C
Catégories apparentées
Description générale
For spectrophotometric measurement of viable cells. Absorbance of converted dye is measured at a wavelength of 540 nm.
Application
In Vitro Toxicology Assay Kit, Neutral Red based has been used:
- to measure the lysosomal enzyme activity in HB8902 cells
- to study the cell viability of human alveolar epithelial cells
- to study the cell viability in fibroblasts
Actions biochimiques/physiologiques
Neutral red (NR) is a red dye that accumulates in the lysosome by actively transferring across the cell membranes. The dye is extracted and the uptake is quantitated by spectroscopy. The NR uptake assay is the most commonly used cytotoxicity test. It is widely applicable in environmental and biomedical studies to estimate the number of viable cells in culture. The NR dye in vitro tissue culture studies is used in assessing viral cytopathogenicity and detection of toxic compounds.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Carc. 1B - Flam. Liq. 3 - Skin Sens. 1
Code de la classe de stockage
3 - Flammable liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
73.4 °F
Point d'éclair (°C)
23 °C
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