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Unfortunately, there is no protocol available for this product. However, see the link below for a publication that may be helpful
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760808/
SRP6371
Aldose Reductase human
recombinant, expressed in E. coli, ≥95% (SDS-PAGE)
Synonyme(s) :
ADR, AKR1B1, ALDR1, ALR2, AR, Aldo-keto reductase family1, member B1
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About This Item
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.32
Produits recommandés
Source biologique
human
Produit recombinant
expressed in E. coli
Essai
≥95% (SDS-PAGE)
Forme
liquid
Poids mol.
35.8 kDa (316 aa, 1-316 aa)
Conditionnement
pkg of 100 μg
Concentration
1 mg/mL
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−70°C
Informations sur le gène
human ... AKR1B1(231)
Description générale
Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily. Recombinant aldose reductase (AKR1B) protein was expressed in Escherichia coli and purified by using conventional chromatography techniques. It is expressed in eye and kidney, but predominant in placenta.[1] The AKR1B1 gene is mapped to human chromosome 7q33.[2]
Actions biochimiques/physiologiques
Aldose reductase (AKR1B1) catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol.[3][4] AKR1B1 is also involved in cytokine-mediated generation of prostaglandin F2α (PGF2α) in preadipocytes.[5] It participates in diabetes-associated mitochondrial disorder and damage via the activation of p53.[6] AKR1B1 is associated with drug resistance mechanism in cancer-derived cell lines. It is also known to mediate cellular processes linked to carcinogenesis such as EMT (epithelial–mesenchymal transition), inflammation and angiogenesis.[7] Aldose reductase mediates glucose metabolism in placenta and osmotic control in eye and kidney.[1]
Forme physique
1 mg/mL in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT and 10% glycerol.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Prostaglandin (PG) F2 alpha synthesis in human subcutaneous and omental adipose tissue: modulation by inflammatory cytokines and role of the human aldose reductase AKR1B1.
Michaud A, et al.
PLoS ONE, 9, e90861-e90861 (2014)
The human aldose reductase AKR1B1 qualifies as the primary prostaglandin F synthase in the endometrium.
Bresson E, et al.
The Journal of Clinical Endocrinology and Metabolism, 96, 210-219 (2011)
The crystal structure of the aldose reductase.NADPH binary complex.
Borhani DW, et al.
The Journal of Biological Chemistry, 267, 24841-24847 (1992)
Expression of the Aldo-Ketoreductases AKR1B1 and AKR1B10 in Human Cancers.
Laffin B and Petrash JM
Frontiers in Pharmacology, 3, 104-104 (2012)
Aldose reductase-mediated phosphorylation of p53 leads to mitochondrial dysfunction and damage in diabetic platelets.
Tang WH, et al.
Circulation, 129, 1598-1609 (2014)
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