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Principaux documents

SRP4901

Sigma-Aldrich

Adiponectin human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonyme(s) :

ACDC, APM-1, Acrp30, AdipoQ, GBP-28

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About This Item

Code UNSPSC :
12352202

Source biologique

human

Produit recombinant

expressed in E. coli

Essai

≥90% (SDS-PAGE)

Forme

lyophilized

Poids mol.

~30 kDa

Conditionnement

pkg of 25 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Impuretés

endotoxin, tested

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... ADIPOQ(9370)

Description générale

Adiponectin (also called Acrp30, AdipoQ) is an adipocyte specific secreted protein that circulates in the plasma. It is induced during adipocyte differentiation and its secretion is stimulated by insulin.[1][2] The protein has a carboxyl-terminal globular domain and an amino-terminal collagen domain.[3] The gene is mapped to human chromosome 3q27.[4] Human adiponectin shares about 83% amino acid identity with that of mouse and about 90% with that of rat.

Application

Adiponectin human has been used to study the effect of adiponectin on androstenedione secretion and oxidative stress in rodent model.[5]

Actions biochimiques/physiologiques

Adiponectin (also called Acrp30, AdipoQ) has antiatherosclerotic and anti-inflammatory properties.[6] It plays a role in various physiological processes such as energy homeostasis and obesity. Plasma levels of adiponectin are reduced in obese humans, and decreased levels are associated with insulin resistance and hyperinsulinemia.[2] It exhibits antidiabetic action by interacting with adiponectin receptors, AdipoR1 and AdipoR2.[3] Low levels of adiponectin are also linked with hypertension and dyslipidemia.[2] Mutation in the gene at position 276G is linked with risk for endometrial cancer.[7]

Forme physique

Lyophilized from a 0.2 μm filtered solution in 25 mM Tris, 0.15 M NaCl, pH 7.4 containing 50 μg of bovine serum albumin (BSA) per 1 μg of adiponectin.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Recontitute in PBS containing at least 0.1% BSA to a concentration of 0.1-1.0 mg/mL. This stock solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Remarque sur l'analyse

Activity of human recombinant adiponectin is measured by its ability to inhibit proliferation of mouse myeloid cell line M1. The ED50 for the effect is typically 1-7 μg/ml.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Plasma concentrations of a novel, adipose-specific protein, adiponectin, in type 2 diabetic patients.
Hotta K
Arteriosclerosis, Thrombosis, and Vascular Biology, 20, 1595-1599 (2000)
Effects of Adiponectin Including Reduction of Androstenedione Secretion and Ovarian Oxidative Stress Parameters In Vivo.
Comim FV
PLoS ONE, 11, e0154453-e0154453 (2016)
Donal O'Shea et al.
PloS one, 5(1), e8660-e8660 (2010-01-29)
Obese individuals who smoke have a 14 year reduction in life expectancy. Both obesity and smoking are independently associated with increased risk of malignancy. Natural killer cells (NK) are critical mediators of anti-tumour immunity and are compromised in obese patients
Eric R Hugo et al.
Environmental health perspectives, 116(12), 1642-1647 (2008-12-17)
The incidence of obesity has risen dramatically over the last few decades. This epidemic may be affected by exposure to xenobiotic chemicals. Bisphenol A (BPA), an endocrine disruptor, is detectable at nanomolar levels in human serum worldwide. Adiponectin is an
Thorhallur I Halldorsson et al.
Environmental health perspectives, 120(5), 668-673 (2012-02-07)
Perfluoroalkyl acids are persistent compounds used in various industrial -applications. Of these compounds, perfluorooctanoate (PFOA) is currently detected in humans worldwide. A recent study on low-dose developmental exposure to PFOA in mice reported increased weight and elevated biomarkers of adiposity

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Lipid Induced Insulin Resistance

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