SRP3269

RANTES human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonyme(s) : CCL5, SIS-delta
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Essai: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Forme: lyophilized
• Puissance: 1.0-10.0 ng/mL
• Poids mol.: 7.8 kDa
• Conditionnement: pkg of 20 μg
• Impuretés: <0.1 EU/μg endotoxin, tested
• Couleur: white
• Numéro d'accès UniProt: P13501
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... CCL5(6352)

Description

Description générale

Regulated on activation, normal T cell expressed and secreted (RANTES) also known as C-C motif chemokine ligand 5 (CCL5) is encoded by the gene mapped to human chromosome 17.[1] RANTES belongs to intercrine or chemokine family of proinflammatory basic polypeptides and is expressed by various hematopoietic and non-hematopoietic cell types involved in homing and migration of effector and memory T cells during acute infections.[2][3] Recombinant human RANTES is a 7.8kDa protein containing 68 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.

Application

RANTES human has been used in following studies:

• In transmigration experiments to determine whether LFA-1 cross-linking could affect peripheral blood T lymphocytes (PBTL) migration in response to the chemokine RANTES.[4]
• Luminol-dependent chemiluminescence assay to determine the effect of RANTES on reactive oxygen species (ROS) production by eosinophils.[5]

Actions biochimiques/physiologiques

Regulated on activation, normal T cell expressed and secreted (RANTES) is a chemoattractant towards monocytes, dendritic cells (DCs), memory T cells (CD4⁺/CD45RO), basophils, and eosinophils.[3] The encoded chemokine protein functions as a ligand for CCR1, CCR3, and CCR5.1 RANTES inhibits the action of R5 strains of HIV-1 via blocking CCR5 receptor. The In1.1C variant of RANTES gene is linked with susceptibility to HIV-1 infection and disease progression. Hence, this can be used as a potent biomarker to determine the prognosis of HIV-1 infection.[1][6]

Forme physique

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable