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SAB1306078

Millipore

ANTI-FLAG TAG (DYKDDDDK) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonyme(s) :

Anti-ddddk, Anti-dykddddk

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

IgG fraction of antiserum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Espèces réactives

all

Concentration

(see Certificate of Analysis)

Technique(s)

western blot: 1:1000

Isotype

IgG

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

FLAG is an octapeptide containing the following amino acid sequence Asp-Try-Lys-Asp-Asp-Asp-Asp-Lys.

Application

ANTI-FLAG TAG (DYKDDDDK) antibody produced in rabbit has been used in western blotting, (1:5000), (1:10000)

Actions biochimiques/physiologiques

FLAG is a widely used epitope tag for immunoaffinity purification of fusion proteins.

Forme physique

Supplied in PBS with 0.09% (W/V) sodium azide

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Code de la classe de stockage

12 - Non Combustible Liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Sunwang Xu et al.
Nature communications, 10(1), 5492-5492 (2019-12-04)
Gemcitabine is the first-line treatment for locally advanced and metastatic gallbladder cancer (GBC), but poor gemcitabine response is universal. Here, we utilize a genome-wide CRISPR screen to identify that loss of ELP5 reduces the gemcitabine-induced apoptosis in GBC cells in
Zhe Wang et al.
JCI insight, 4(1) (2019-01-11)
Deposition of amyloid-β protein (Aβ) to form neuritic plaques is the characteristic neuropathology of Alzheimer's disease (AD). Aβ is generated from amyloid precursor protein (APP) by β- and γ-secretase cleavages. BACE1 is the β-secretase and its inhibition induces severe side
B L Brizzard et al.
BioTechniques, 16(4), 730-735 (1994-04-01)
The FLAG epitope is an eight amino acid peptide (AspTyrLysAspAspAspAspLys) that is useful for immunoaffinity purification of fusion proteins. A monoclonal antibody (anti-FLAG M1) that binds the FLAG epitope in a calcium-dependent manner and requires an N-terminal FLAG sequence has
Feiran Zhang et al.
Human molecular genetics, 27(22), 3936-3950 (2018-08-15)
N6-methyladenosine (m6A) is the most prevalent internal modification of mammalian messenger RNAs (mRNAs) and long non-coding RNAs. The biological functions of this reversible RNA modification can be interpreted by cytoplasmic and nuclear 'm6A reader' proteins to fine-tune gene expression, such
Renxu Chang et al.
Nature communications, 9(1), 3486-3486 (2018-08-30)
Loss of WW domain-containing oxidoreductase (Wwox) expression has been observed in breast cancer (BC). However, its regulatory effects are largely unknown, especially in triple-negative breast cancer (TNBC). Herein, gene expression profiling revealed that JAK/STAT3 pathway was one of the most
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