R6148
RNase A solution
Synonyme(s) :
GenElute™ RNAse A
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About This Item
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.52
Produits recommandés
Source biologique
bovine pancreas
Forme
liquid
Activité spécifique
1500 units/mL
3000 units/mL
Concentration
20 mg/mL in Tris buffer
Technique(s)
DNA purification: suitable
Température de stockage
room temp
Informations sur le gène
bovine ... RNASE1(282340) , RNASE1(280930)
Description générale
Ribonuclease A (RNase A), a protein composed of three α helices and seven β strands arranged in two "lobes” holds significance as the prototypical domain-swapped protein. It is known for its diverse oligomeric states. The RNase A family derives its name from bovine pancreatic RNase A and is specific to vertebrates. In humans, this family encompasses eight functional members, showcasing its biological relevance.
For sample preparation in GenElute™ kits.
For sample preparation in GenElute™ kits.
Application
RNase A solution has been used:
- in apoptosis assay of Hep-2 cells
- for the colorimetric quantification of methylated DNA
- for DNA and RNA extraction
- in DNA laddering analysis
- in cell cycle analysis
- as a component in phosphate-buffered saline (PBS) to incubate cells for flow cytometry
Actions biochimiques/physiologiques
Ribonuclease A or bovine pancreatic ribonuclease A (RNase A) enzyme is an endoribonuclease that displays a specific ability to cleave single-stranded RNA on the 3′ side of pyrimidine residues. This endonuclease with 124 amino acids plays a key role in gene duplication, cellular metabolism, and cancer therapy. Its overexpression is closely associated with several types of cancer. Hence, RNase A has the potential to be a promising new biomarker for the diagnosis of malignant tumors and is also used as a specific target for new drug discovery. RNase A is commonly used for the isolation of plasmid DNA.
Notes préparatoires
For usage requirements, please refer to the kit procedure.
Informations légales
GenElute is a trademark of Sigma-Aldrich Co. LLC
Préparation d'échantillons
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Bovine Pancreatic Ribonuclease A: Oxidative and Conformational Folding Studies
Scheraga HA, et al.
Methods in Enzymology, 341, 189-221 (2001)
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