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MBD0052

Sigma-Aldrich

Hemin solution

Ready-made microbial growth medium supplement, 0.5 g/L

Synonyme(s) :

Chlorohemin solution, ChloroprotoporphyrinIX iron(III) solution, Ferriprotoporphyrin IX chloride Solution

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About This Item

Code UNSPSC :
41106212
Nomenclature NACRES :
NA.21

Niveau de qualité

200

Forme

liquid

Utilisation

supplement for growth media

Concentration

0.5 g/L

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

Hemin is a pro-oxidant, hemolysis derived-product. It is essential for the growth of heme-deficient organisms such as trypanosomatids. Hemin readymade solution is used as a culture-medium supplement for fastidious anaerobic microorganisms, shown to enhance the growth of many anaerobes including Bacteroides species and certain gram-positive non-spore forming organisms. It is present as a key ingredient compound in common anaerobic growth media, such as Brucella broth, Thioglycolate broth and Reinforced Clostridial Medium.

Application

Hemin solution may be used as a supplement in brain heart infusion-yeast extract-hemin (BHI-YH) medium to determine cholesterol-to-coprostanol reducing activity.

Caractéristiques et avantages

  • Convenient readymade solution to speed up media preparation
  • Sterile solution
  • Optimal mixture for growth of fastidious bacteria as well as diversified culturing of microbial samples

Autres remarques

  1. For 100 ml of medium preparation, it is recommended to add 1 mL hemin ready-made solution after autoclaving the medium, to achieve a final concentration of 5 microgram/ml. The optimal concentration of the supplement may vary for each strain.
  2. The solution is classified as light sensitive. 2. The solution should not be heated and must be added aseptically to autoclaved media cooled to 50-55°C. 3.
  3. Select strains which show turbid growth (24-48 hr.) with Hemin supplementation: Porphyromonas gingivalis, Bifidobacterium longum, Clostridium perfringens, Bacteroides vulgatus and Fusobacterium nucleatum.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

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Consulter la Bibliothèque de documents

María Elisa Lombardo et al.
Analytical biochemistry, 341(2), 199-203 (2005-05-24)
Hemin chlorides exhibit two absorption maxima in the Soret region, one at about 360-380 nm (S' band) and the other between 400 and 430 nm (S band). We present here a simple and fast spectrophotometric assay to determine concentration of
Manual of clinical microbiology
Murray, P.R., E.J. Baron, M.A.Pfaller, F.C. Tenover, and R.H.Yolken
ASME 2013 Gas Turbine India Conference. American Society of Mechanical Engineers, 2013., 2, 94305-95250 (2006)
Sânzio Silva Santana et al.
Frontiers in immunology, 11, 1488-1488 (2020-08-09)
The excessive release of heme during hemolysis contributes to the severity of sickle cell anemia (SCA) by exacerbating hemoglobin S (HbS) autoxidation, inflammation and systemic tissue damage. The present study investigated the effect of hydroxyurea (HU) on free radical neutralization
Philippe Gérard et al.
Applied and environmental microbiology, 73(18), 5742-5749 (2007-07-10)
The microbial community in the human colon contains bacteria that reduce cholesterol to coprostanol, but the species responsible for this conversion are still unknown. We describe here the first isolation and characterization of a cholesterol-reducing bacterium of human intestinal origin.
Hemin and vitamin K compounds as required factors for the cultivation of certain strains of Bacteroides melaninogenicus.
R J GIBBONS et al.
Journal of bacteriology, 80(2), 164-170 (1960-08-01)

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