Alcohol dehydrogenase (ADH) is an oxidoreductase which catalyzes the interconversion of alcohols and aldehydes or ketones. ADH is important in humans and other organisms for the break down of alcohols which may otherwise be toxic. In yeast and some bacteria, ADHs catalyze the opposite reaction and produce alcohol as part of fermentation.
Caractéristiques et avantages
Linear detection range: 0.4 to 80 U/L for 30 minute reaction. Detection Limit of 0.1 U/L for 120 minute reaction.
Compatible with high-throughput handling systems.
Adéquation
The kit is suitable for alcohol dehydrogenase activity determination in biological samples (e.g. plasma, serum, urine, tissue and culture media.)
Principe
The non-radioactive, colorimetric Alcohol Dehydrogenase (ADH) Assay Kit is based on the reduction of the tetrazolium salt MTT in a NADH coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.
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