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Principaux documents

MAK194

Sigma-Aldrich

Lipid (Oil Red O) Staining Kit

Sufficient for 2 96-well plates

Synonyme(s) :

Oil Red O Lipid Staining Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.25

Maladie(s) pertinente(s)

endocrinological disorders, diabetes; obesity; cardiovascular diseases

Température de stockage

room temp

Description générale

Lipid droplets (LDs) are dynamic, ubiquitously present lipid-storage organelles, predominantly present in the adipocytes. Triglycerides, neutral lipids, and cholesterol esters stored in LDs are the largest sources of energy. The presence of excess LDs in adipocytes results in obesity and obesity-linked pathologies such as dyslipidemia and diabetes type.

Application

Lipid (Oil Red O) Staining Kit has been used to stain lipids[1] and adipocytes[2] in samples.

Adéquation

This kit is suitable for staining neutral lipids in two 96 well plates, two 6 well plates, or four 100 mm culture dishes.

Principe

The Lipid (Oil Red O) Staining Kit is suitable for selective staining and detection of neutral lipids in cultured cells. Hematoxylin included in the kit stains the nuclei of the cells.

Composants de kit seuls

Réf. du produit
Description
  • PBS
  • Formalin (10%)
  • Oil Red O
  • Hematoxylin

Pictogrammes

Skull and crossbonesHealth hazard
GHS06,GHS08

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
8A11K05800
8J27K05800
8F14K05800
8C04K05800
8A26K05800

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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Kefeng Yang et al.
Xenotransplantation, 24(5) (2017-07-19)
Survival and longevity of xenotransplants depend on immune function and ability to integrate energy metabolism between cells from different species. However, mechanisms for interspecies cross talk in energy metabolism are not well understood. White adipose tissue stores energy and is
CD36 mediates lipid accumulation in pancreatic beta cells under the duress of glucolipotoxic conditions: Novel roles of lysine deacetylases.
Khan S and Kowluru A
Biochemical and Biophysical Research Communications, 495(3), 2221-2226 (2018)
Yohei Nomura et al.
Frontiers in physiology, 12, 675724-675724 (2021-07-06)
We previously reported that histone deacetylase 6 (HDAC6) has an important role in endothelial cell (EC) function in vitro. However, whether HDAC6 plays a role in atherogenesis in vivo and the mechanism(s) that control HDAC6 activity/expression in response to atherogenic
Aldehyde dehydrogenase 1 a1 regulates energy metabolism in adipocytes from different species.
Yang K, et al.
Xenotransplantation, 24(5), e12318-e12318 (2017)
Jonathan J Hernandez et al.
Journal of translational medicine, 19(1), 149-149 (2021-04-16)
Mesenchymal stem cells derived from human umbilical cord (hUC-MSCs) have immunomodulatory properties that are of interest to treat novel coronavirus disease 2019 (COVID-19). Leng et al. recently reported that hUC-MSCs derived from one donor negatively expressed Angiotensin-Converting Enzyme 2 (ACE2)
Afficher tous les articles scientifiques apparentés

Questions

1–3 sur 3 questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 réponse

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 réponse

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. What are the methods for removing red stains from glassware from kit MAK194?

    1 réponse

    1. For cleaning glassware from kit MAK194, it is suggested to rinse the glassware with ethanol and then scrub it with warm tap water and soap to remove the red staining caused by the Oil Red O solution. Following these steps with a standard wash in a lab dishwasher should yield optimal results. It's important to note that the longer the solution remains in the glassware, the more difficult it becomes to remove. Therefore, it is recommended to prepare special staining solutions in one-use plastic bottles to avoid this issue.

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