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Key Documents

L9031

Sigma-Aldrich

Lipase from Mucor miehei

lyophilized powder, ≥4,000 units/mg solid (using olive oil)

Synonyme(s) :

Triacylglycerol acylhydrolase, Triacylglycerol lipase

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About This Item

Numéro CAS:
Numéro de classification (Commission des enzymes):
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Forme

lyophilized powder

Niveau de qualité

Activité spécifique

≥4,000 units/mg solid (using olive oil)

Température de stockage

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

Clé InChI

QWZUIMCIEOCSJF-CHHCPSLASA-N

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Description générale

Lipase is produced by several animals, microbes, and plants. Lipase from Mucor sp. is a fungal lipase. It is a member of the serine hydrolase family.

Application

Lipase from Mucor miehei can be used to drive the synthesis of flavor esters.
Lipase from Mucor miehei has been used in a study to assess the nonthermal effect of microwave irradiation in nonaqueous enzymatic esterification. It has also been used in a study to investigate the hydrolysis of virgin coconut oil using immobilized lipase in a batch reactor.
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Actions biochimiques/physiologiques

Mucor miehei lipase hydrolizes triglycerides and catalyzes the synthesis of esters from glycerol and long-chain fatty acids in vitro. Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).
Microbial lipase has a wide industrial application due to its stability, broad substrate specificity, and selectivity. It may be used in the synthesis of biosurfactants and finds application in organic chemical processing, dairy industry, oleochemical industry, detergent formulations, paper, cosmetics, nutrition, and the pharmaceutical industry.

Définition de l'unité

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.7 at 37 °C using olive oil.

Remarque sur l'analyse

Protein determined by biuret.

Pictogrammes

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Conseils de prudence

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Lee Suan Chua et al.
Enzyme research, 2012, 542589-542589 (2012-09-07)
Hydrolysis of virgin coconut oil (VCO) had been carried out by using an immobilised lipase from Mucor miehei (Lipozyme) in a water-jacketed batch reactor. The kinetic of the hydrolysis was investigated by varying the parameters such as VCO concentration, enzyme
Design and synthesis of an affinity probe that targets caspases in proteomic experiments
Liau, M-L, et al
Tetrahedron Letters, 44, 1043-1046 (2003)
Hui-da Wan et al.
Applied biochemistry and biotechnology, 166(6), 1454-1462 (2012-01-21)
Microwave has nonthermal effects on enzymatic reactions, mainly caused by the polarities of the solvents and substrates. In this experiment, a model reaction with caprylic acid and butanol that was catalyzed by lipase from Mucor miehei in alkanes or arenes
Agnieszka Stobiecka
Journal of photochemistry and photobiology. B, Biology, 80(1), 9-18 (2005-06-21)
Steady-state and time-resolved fluorescence-quenching measurements have been performed to study multitryptophan lipase from filamentous fungus Rhizomucor miehei. Using the steady-state acrylamide fluorescence quenching data and the fluorescence-quenching-resolved-spectra (FQRS) method, the total emission spectrum of native ("closed-lid") lipase has been decomposed
H S Ryu et al.
Applied microbiology and biotechnology, 70(3), 321-326 (2005-08-10)
A Photobacterium strain, M37, showing lipolytic activity, was previously isolated from an intertidal flat of the Yellow Sea in Korea and identified as Photobacterium lipolyticum sp. nov. In the present study, the corresponding gene was cloned using the shotgun method.

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