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H5041

Sigma-Aldrich

Cystatin C human

recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

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About This Item

Numéro MDL:
MFCD00163390
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

Source biologique

human

Niveau de qualité

100

Produit recombinant

expressed in HEK 293 cells

Pureté

≥95% (SDS-PAGE)

Forme

lyophilized powder

Puissance

≤5 μM IC50

Qualité

endotoxin tested

Poids mol.

dimer 12-13 kDa (non-glycosylated)

Conditionnement

pkg of 10 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Technique(s)

cell culture | mammalian: suitable

Impuretés

≤1 EU/mg

Numéro d'accès UniProt

P01034

Température de stockage

−20°C

Informations sur le gène

human ... CYTC(1471)

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Description générale

HumanKine human Cystatin C is expressed as a non-glycosylated monomer in human HEK 293 cells. Cystatin C belongs to the cystatin superfamily. Two isoforms (pI = 7.8, 9.2) of native Cystatin C are found in human urine differentiated by the elimination of small basic peptides or amino acids from the N-terminal end of the protein. This protein is coded by a housekeeping gene, CST3 located on human chromosome 20p11.21 and is generated by nucleated cells.

Application

Cystatin C human has been used as a standard in western blot assay. It has also been used to study its effect on the endopeptidase activity of activated Trichobilharzia regenti isoform of cathepsin B1 peptidase (TrCB1).

Actions biochimiques/physiologiques

Cystatin C is an inhibitor of cysteine proteases including cathepsin B which has been identified as the most important β-amyloid-degrading enzyme. Measurement of cystatin C in serum is replacing creatinine as an indicator of kidney function (glomerular filtration rate, GFR). Studies show its role in predicting new-onset or deteriorating cardiovascular disease. It also seems to play a role in brain disorders involving amyloid, such as Alzheimer′s disease. Cystatin C inhibits transforming growth factor β signaling in normal and cancer cells.

Forme physique

Lyophilized from a 0.2 μm filtered solution of 1×PBS

Remarque sur l'analyse

The inhibitory function of cystatin c on papain′s protease activity was measured by a colorimetric assay using L-BAPA as substrate. IC50 value was measured at 5 to 20 μg/mL (0.3 to 1.5 μM) with a range of 1.56 μg/mL to 50 μg/mL cystatin C in presence of 0.55 μM papain and 0.44 μM L-BAPA.

Informations légales

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Assessment of glomerular filtration rate in acute and chronic settings
National Kidney Foundation Primer on Kidney Diseases, 26-32 (2014)
M S N Murty et al.
Indian journal of nephrology, 23(3), 180-183 (2013-07-03)
In patients with acute kidney injury (AKI), serum creatinine level does not increase until moderate to severe reduction in glomerular filtration rate (GFR) occurs. Thus its use for estimating GFR in early AKI delays detection of kidney damage and making
Assessing kidney function
Chronic Renal Disease, 31-42 (2015)
Cystatin C Antagonizes Transforming Growth Factor $\beta$ Signaling in Normal and Cancer Cells11Start-up fund from the National Jewish Medical and Research Center and by a grant from the Elsa U. Pardee Foundation to W. Schiemann.
Sokol JP and Schiemann WP
Molecular Cancer Research, 2, 183-195 (2004)
Jean-Charles Lafarge et al.
Biochimie, 92(11), 1580-1586 (2010-04-27)
Given the increasing prevalence of human obesity worldwide, there is an urgent need for a better understanding of the molecular mechanisms linking obesity to metabolic and cardiovascular diseases. Our knowledge is nevertheless limited regarding molecules linking adipose tissue to downstream
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