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G1N10

Sigma-Aldrich

GenElute™ Mammalian Genomic DNA Miniprep Kits

sufficient for 10 purifications

Synonyme(s) :

Mammalian Genomic DNA Miniprep, Gen Elute

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About This Item

Code UNSPSC :
41105501
Nomenclature NACRES :
NA.55

Utilisation

sufficient for 10 purifications

Température de stockage

15-25°C

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Catégories apparentées

Description générale

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.

Isolate pure genomic DNA from cultured cells, tissues (including rodent tails), and fresh whole blood or white blood cells. The kit combines the advantages of silica binding with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Application

The purified genomic DNA is ready for immediate use in downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • sequencing reactions
  • cloning
  • ligation

Caractéristiques et avantages

  • Expected yield: 25 μg from 2 x 106 cultured cells; 30 μg from 25 mg of tissue
  • Elution volume: 200 - 400 μl
  • Time required: 20 min after lysis
  • A260/A280 ratio: 1.6 - 1.9
  • Mechanical homogenization required: No

Principe

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Autres remarques

For additional information, please see www.sigma-aldrich.com/genomicdna.

Informations légales

GenElute is a trademark of Sigma-Aldrich Co. LLC

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • C2112Column Preparation SolutionFDS
  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biologyFDS
  • R6148RNase A solutionFDS

En option

Réf. du produit
Description
Tarif

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

A9434

Chlorure d′ammonium, for molecular biology, suitable for cell culture, ≥99.5%

P2308

Protéinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology

E1510

Ethidium bromide solution, BioReagent, for molecular biology, 10 mg/mL in H2O

A9539

Agarose, BioReagent, for molecular biology, low EEO

G1N70

GenElute™ Mammalian Genomic DNA Miniprep Kits, sufficient for 70 purifications

D9281

Lambda DNA EcoR I Hind III Digest, for DNA electrophoresis

L3287

Escort™ IV Transfection Reagent, Lipid reagent for transient and stable transfection of mammalian and insect cells.

T6400

Tampon Tris-borate-EDTA, 5× Concentrate

G1N350

GenElute™ Mammalian Genomic DNA Miniprep Kits, sufficient for 350 purifications

R6148

RNase A solution

C2112

Column Preparation Solution

93221-001

RNAstable®, Tube Kit, for room temp preservation of RNA

T0956

Sigma Universal Transfection Reagent, Polymer reagent for transfecting common mammalian cell lines

SRE0005

Protéinase K from Tritirachium album, Reagents designed and manufactured under current ISO 13485 certification.

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Point d'éclair (°F)

483.8 °F

Point d'éclair (°C)

251 °C

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Contenu apparenté

Mammalian Genomic DNA Miniprep Kit

GenElute Mammalian Genomic DNA Purification Kit isolates pure DNA from mammalian cells using a silica-based membrane in spin columns.

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