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F9765

Filipin complex

powder, ≥70% (UV)

Synonyme(s) :

Filimarisin

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25 mg

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A propos de cet article

Numéro CAS:
11078-21-0
UNSPSC Code:
12352202
NACRES:
NA.77
MDL number:
MFCD00131073

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Nom du produit

Filipin complex from Streptomyces filipinensis, ≥70% (UV)

biological source

Streptomyces sp. (Streptomyces filipinensis)

Quality Segment

assay

≥70% (UV)

form

powder

technique(s)

protein detection: suitable

solubility

methanol: 9.80-10.20 mg/mL, clear to hazy, yellow to very deep greenish-yellow

antibiotic activity spectrum

fungi

mode of action

cell membrane | interferes

storage temp.

−20°C

InChI

1S/C35H58O11/c1-4-5-11-16-31(42)34-33(44)22-29(40)20-27(38)18-25(36)17-26(37)19-28(39)21-32(43)23(2)14-12-9-7-6-8-10-13-15-30(41)24(3)46-35(34)45/h6-10,12-15,24-34,36-44H,4-5,11,16-22H2,1-3H3/b7-6+,10-8+,12-9+,15-13+,23-14+/t24-,25+,26-,27+,28-,29+,30+,31-,32+,33+,34-/m1/s1

InChI key

IMQSIXYSKPIGPD-YQRUMEKGSA-N

General description

Chemical structure: macrolide

Application

Filipin complex from Streptomyces filipinensis has been used as a profluorescence probe to detect the free cholesterol in cell.

Biochem/physiol Actions

Filipin is a polyene macrolide antibiotic and antifungal. The antifungal mechanism of action is unclear but may be due to altering membrane permeability and associated functions via binding to membrane sterols. Filipin binds to membrane sterols such as cholesterol, and it both inhibits prion protein (PrP) endocytosis and causes the release of PrP from the plasma membrane.
Filipin is a polyene macrolide antibiotic and antifungal. The antifungal mechanism of action is unclear but may be due to altering membrane permeability. Filipin binds to model crystalline phase lipid bilayers containing cholesterol, and to liquid phase bilayers with or without cholesterol, in an orientation that forms membrane pores.It both inhibits prion protein (PrP) endocytosis and causes the release of PrP from the plasma membrane.

Other Notes

Filipin contains 8 isomeric components, with filipin III as the major component.

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Cet article
S4400K4000K4013
Filipin complex from Streptomyces filipinensis ≥70% (UV)

Sigma-Aldrich

F9765

Filipin complex from Streptomyces filipinensis

Staurosporine from Streptomyces sp. ≥98% (HPLC), film

Sigma-Aldrich

S4400

Staurosporine from Streptomyces sp.

Kanamycine sulfate from Streptomyces kanamyceticus Animal Component-free

Sigma-Aldrich

K4000

Kanamycine sulfate from Streptomyces kanamyceticus

Kasugamycin hydrochloride from Streptomyces kasugaensis

Sigma-Aldrich

K4013

Kasugamycin hydrochloride from Streptomyces kasugaensis

description

≥70% (UV)

description

≥98% (HPLC), film

description

Animal Component-free

description

-

assay

≥70% (UV)

assay

≥98% (HPLC)

assay

-

assay

-

biological source

Streptomyces sp. (Streptomyces filipinensis)

biological source

Streptomyces sp.

biological source

Streptomyces kanamyceticus

biological source

Streptomyces kasugaensis

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

form

powder

form

film

form

powder

form

powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

solubility

methanol: 9.80-10.20 mg/mL, clear to hazy, yellow to very deep greenish-yellow

solubility

ethanol: soluble, DMSO: soluble, methanol: soluble, H2O: insoluble

solubility

H2O: 50 mg/mL, clear, colorless to faintly yellow

solubility

-

Pictogrammes

Exclamation mark
GHS07

Mot-clé

Warning

Codes de danger

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Classe de stockage

11 - Combustible Solids

Que se passe-t-il ?

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number

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Questions

1–6 sur 6 questions  

  1. What is the stability of filipin when dissolved in DMSO and stored at -20 degrees C (how long can it be stored like this)?

    1 réponse

    1. This product has not been tested for stability in solution, however product SAE0088 is a prepared 5/mg/mL solution in DMSO. This product is stable for at least 2 years at -20 deg. C. but does contain a proprietary stabilizer to enhance stability. See the link below to the product information sheet on the SAE0088 solution:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/973/858/sae0088pis.pdf

      Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a Certificate of Analysis for the prepared solution which includes the retest date:
      https://www.sigmaaldrich.com/product/sigma/f9765#product-documentation

      Utile ?

  2. Which filipin is better to visualize cholesterol in hela cells by immunofluorescence - F9765 or F4767?

    1 réponse

    1. Both F9765 and F4767 are suitable for determining cholesterol in cells. The F4767, Filipin III, is purified from Filipin. As it has a higher purity, it may offer a higher signal-to-noise ratio resulting in less background. These product have not been compared in side by side testing. It may be helpful to view the peer-reviewed knowledge articles to help with the appropriate selection:

      https://www.sigmaaldrich.com/search/f9765?focus=papers&page=1&perpage=30&sort=relevance&term=f9765&type=citation_search

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  3. What is the difference between Filipin products F9765 and F4767?

    1 réponse

    1. The product F9765 consists of a filipin complex. It is composed of 8 isomeric components, of which filipin III is the major component in the complex. F4767 consists of filipin III alone and is considered to be a better choice for staining.

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  4. How should Product F9765, Filipin complex from Streptomyces filipinensis, be solubilized?

    1 réponse

    1. This product has low solubility in aqueous buffers. It should initially be reconstituted in DMSO at 1 mg/mL and then diluted into saline or an aqueous buffer of choice.

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  5. What is the stability of stock solutions Product F9765, Filipin complex from Streptomyces filipinensis?

    1 réponse

    1. Solutions of filipin in aqueous or organic solvents are not considered to be stable and it is recommended that they be prepared fresh and not stored as stock solutions.

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  6. What is the Department of Transportation shipping information for this product?

    1 réponse

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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