EPI023
Adenosine Deaminase Activity Assay Kit (Colorimetric)
sufficient for 100 colorimetric tests
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About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.25
Produits recommandés
Utilisation
sufficient for 100 colorimetric tests
Méthode de détection
colorimetric
Température de stockage
−20°C
Description générale
Adenosine Deaminase (ADA) (E.C. 3.5.4.4.) is an enzyme that catalyzes the conversion of adenosine and 2′-deoxyadenosine to inosine and 2′-deoxyinosine. Adenosine Deaminase is widely distributed in various tissues and cells. There are two isoforms, ADA1 and ADA2. ADA1 is widely expressed in most cells in the body, particularly in lymphocytes and macrophages. It is present in the cytosol, nucleus and found associated with dipeptidyl peptidase-4 on the cell membrane. ADA2 was first found in the spleen but is predominantly found in the plasma and serum. Increased serum ADA levels are found in certain infectious diseases such as tuberculosis and various liver diseases such as acute hepatitis, alcoholic hepatic fibrosis, chronic active hepatitis to name a few. Adenosine Deaminase is also a marker for T-lymphocyte proliferation.
Adéquation
Suitable for the detection of Adenosine Deaminase (ADA) activity in purified recombinant protein, nuclear extract and cell and tissue lysate
Principe
Inosine formed from the breakdown of adenosine is converted to uric acid with ADA Convertor and ADA Developer. The uric acid is measured at A293 nm. The kit measures total activity of adenosine deaminase with limit of quantification of 1 mU of recombinant Adenosine Deaminase.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 4 Dermal - Aquatic Chronic 3 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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