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Merck
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Principaux documents

EC11303

Sigma-Aldrich

Escherichia coli

Strain B, lyophilized cells

Synonyme(s) :

E. coli

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10 G
268,00 €
25 G
587,00 €

268,00 €

Date d'expédition estimée le12 avril 2025

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10 G
268,00 €
25 G
587,00 €

About This Item

Numéro MDL:
MFCD00131026
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.83

268,00 €

Date d'expédition estimée le12 avril 2025

Niveau de qualité

200

Stérilité

non-sterile (not processed or packaged aseptically)

Forme

lyophilized cells

Adéquation

not intended for starter culture

Température de stockage

−20°C

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Description générale

Escherichia coli is a non-pathogenic, facultative anaerobic flora present in the intestine of human.[1] It belongs to the genus, Escherichia, which consists of motile gram-negative bacilli.[1]

Application

Escherichia coli has been used:
  • in the isolation of DNA from lyophilized cell[2]
  • in the preparation of crude cell lysate for developing scalable purification method for recombinant protein A[3]
  • as test proteome for comparative electrophoresis[4]

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000324914
0000305774
0000231256
0000220774
048M4031V

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Diarrheagenic Escherichia coli
Nataro JP and Kaper JB
Clinical Microbiology Reviews, 11(1), 142-201 (1998)
Accelerated recombinant protein purification process development: Automated, robotics-based integration of chromatographic purification and analysis
Londo T, et al.
Journal of Chromatography A, 798(1), 73-82 (1998)
Severe atopic dermatitis is associated with a high burden of environmental Staphylococcus aureus
Leung AD, et al.
Clinical and Experimental Allergy, 38(5), 789-793 (2008)
Horizontal comparative fluorescence two-dimensional gel electrophoresis for improved spot coordinate detection
Hanneken M, et al.
Electrophoresis, 35(8), 1118-1121 (2014)
A D Leung et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 38(5), 789-793 (2008-03-18)
About 90% of patients with atopic dermatitis (AD) are colonized with Staphylococcus aureus. S. aureus worsens AD by secreting superantigens and structural molecules within the cell wall that induce skin inflammation. Therefore, S. aureus in the home may contribute to
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Questions

1–2 sur 2 questions  

  1. Could you provide the reconstitution method and concentration for product EC11303 under the brand SIGMA?

    1 réponse

    1. The cells in this product are expected to be viable and may grow in culture, but it's important to note that the product is not intended for culture use. The material is not handled aseptically during manufacturing, so it is likely that other organisms would be present. The main purpose of this product is to serve as a biomass, specifically for the extraction of biomolecules such as DNA and lipids. It's important to understand that there is no guarantee of viability or purity. If the end user wishes to reconstitute the product for culture, they should add 0.3 to 0.4 ml of suitable liquid medium/broth to the freeze-dried material and mix it well. Then, they should transfer the total mixture to a culture tube or plate containing 5 - 6 mL medium/broth. It's worth noting that there is no cell count estimate available for this product.

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  2. What is the method of reconstitution for this product and what is the concentration?

    1 réponse

    1. These cells are expected to be viable and may grow in culture. However, the product is not intended for culture use. The material is not handled aseptically during manufacturing, therefore other organisms would likely be present. The product is intended to serve as a biomass, i.e., for the extraction of biomolecules such as DNA and lipids. There is no guarantee of viability or purity. Should the end user wish to reconstitute the product for culture, add to the freeze-dried material 0.3 to 0.4 ml of suitable liquid medium/broth and mix well. Transfer the total mixture to a culture tube or plate containing 5 - 6 mL medium/broth. There is no cell count estimate available.

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