The recommendation to store this product at -20°C is due to the presence of 50% glycerol, which lowers its freezing point to approximately -23°C. Consequently, it should not freeze at -20°C; if it does, it may indicate that the freezer is set too low, not properly calibrated, or that the glycerol ratio is incorrect. If the material is frozen at or below -23°C, or if it contains less or no glycerol, the gel could be damaged, become fragile, and fail to perform as expected.
E6406
EZview™ Red Glutathione Affinity Gel
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1 ML
340,00 €
5 X 1 ML
705,00 €
340,00 €
Date d'expédition estimée le02 juin 2025
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Changer de vue
1 ML
340,00 €
5 X 1 ML
705,00 €
About This Item
340,00 €
Date d'expédition estimée le02 juin 2025
Produits recommandés
Classe(s) chimique(s) de l'analyte
proteins (GST)
Technique(s)
immunoprecipitation (IP): suitable
protein purification: suitable
Température de stockage
2-8°C
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Description générale
EZviewä Red Glutathione Affinity Gel is a highly visible, red colored glutathione agarose affinity gel. The affinity resin is composed of glutathione covalently attached through the sulfur to epoxy-activated 4% cross-linked agarose beads, resulting in a 12-atom spacer.
Application
The product is used for affinity capture, molecular pull-down, or IP of proteins containing glutathione binding sequences, such as glutathione S-transferase, glutathione peroxidase and glyoxalase I.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview™ resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.
Caractéristiques et avantages
- Increased visibility - Red color reduces risk of incidental aspiration
- Improved recovery of target protein by reduced accidental loss
- Higher reproducibility - More consistent yields
Forme physique
1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
Informations légales
EZview is a trademark of Sigma-Aldrich Co. LLC
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
P L Hallauer et al.
BMC genetics, 1, 1-1 (2000-10-19)
Versatile transgenic manipulation of skeletal muscle requires knowledge of the expression profiles of diverse promoter/enhancer elements in the transcriptionally specialized fiber types of which muscle is composed. "Universal" viral promoters/enhancers, e.g., cytomegalovirus IE1 (CMV IE1), are of interest as reagents
S K Jang et al.
Journal of virology, 62(8), 2636-2643 (1988-08-01)
Picornavirus RNAs are uncapped messengers and have unusually long 5' nontranslated regions (5'NTRs) which contain many noninitiating AUG triplets. The translational efficiency of different picornavirus RNAs varies between different cell-free extracts and even in the same extract, such as micrococcal
S Andersson et al.
The Journal of biological chemistry, 264(14), 8222-8229 (1989-05-15)
The conversion of cholesterol into bile acids in the liver represents the major catabolic pathway for the removal of cholesterol from the body. In this complex biosynthetic pathway, at least 10 enzymes modify both the ring structure and side chain
D R Thomsen et al.
Proceedings of the National Academy of Sciences of the United States of America, 81(3), 659-663 (1984-02-01)
The DNA templates containing immediate early (IE) genes of human cytomegalovirus (CMV) were transcribed in vitro by using a HeLa cell extract. When IE region 1, 2, and 3 were used, transcription was detected qualitatively only from IE region 1.
Contenu apparenté
Étudiez les interactions protéine-protéine in vitro avec des essais de pull-down (interactions protéines/protéines) reposant sur des méthodes d'affinité, de pull-down avec GST, de co-immunoprécipitation et de double purification par affinité du type méthode TAP.
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
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Why should E6406 be stored at -20°C, and what could happen if it is stored at a colder temperature?
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