E0287

Carboxylesterase 1 isoform b human

recombinant, expressed in baculovirus infected BTI insect cells

• Synonyme(s) : Carboxylesterase 1 human, carboxylesterase, esterase
• Numéro de classification (Commission des enzymes): 3.1.1.1 (BRENDA, IUBMB)
• Numéro CE : 232-773-7
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Produit recombinant: expressed in baculovirus infected BTI insect cells
• Niveau de qualité: 200
• Forme: liquid
• Activité spécifique: ≥500 units/mg protein
• Concentration: 5 mg/mL
• Poids: (0.5 ml)
• Conditions d'expédition: dry ice
• Température de stockage: −70°C

Description

Description générale

Carboxylesterase (CE) belongs to serine hydrolase family.[1] It is widely present in the human body. Five isoforms of carboxylesterase is present in humans.[2] It is encoded by CES1 gene mapped to 16q22.2 in human chromosome.[3]

Carboxylesterase 1 is a member of a large multigene carboxylesterase family. These enzymes are responsible for the hydrolysis of ester- and amide-bond-containing drugs such as cocaine and heroin. They also hydrolyze long-chain fatty acid esters and thioesters. This enzyme is known to hydrolyze aromatic and aliphatic esters and is necessary for cellular cholesterol esterification. It may also play a role in detoxification in the lung and/or protection of the central nervous system from ester or amide compounds.

Application

Carboxylesterase 1 isoform b human has been used in the reaction to convert Amplex red to resorufin in horse radish peroxidase and oxygen independent manner.[1] It has also been used in the development of an emission ratiometric two-photon probe (SE1) for quantitation in situ.[2]

Delivers high catalytic activity, ideal for robust high-throughput screening assays including drug-drug interaction studies, and pharmacokinetic studies for evaluating pro-drugs and non-CYP pathways of elimination.

Actions biochimiques/physiologiques

Carboxylesterase (CE) is implicated in drug metabolism in liver and hepatic steatosis. Deficiency of CE in humans causes obesity, hyperlipidemia, insulin insensitivity, and cancer.[2]

Définition de l'unité

One unti will hydrolyze one nanomole of 4-nitrophenyl acetate per minute at pH 7.4 at 37°C

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable