For a list of components, see the link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/747/307/d8437for.pdf
D8437
DMEM/F-12
With ʟ-glutamine, HEPES, sodium pyruvate and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham
Synonyme(s) :
DME/F-12, mélange 1/1
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Milieu de Eagle modifié par Dulbecco (DMEM)/Mélange nutritif F-12 de Ham, With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Segment
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
glucose: 3.15 g/L
HEPES: yes
L-glutamine: yes
phenol red: yes
sodium pyruvate: 0.055 g/L
shipped in
ambient
storage temp.
2-8°C
General description
Le milieu de Eagle modifié par Dulbecco (DMEM), un milieu de culture cellulaire régulièrement employé, est un milieu riche en sources d'énergie comprenant du glucose, des vitamines et des acides aminés.[1] Le mélange 1/1 de milieu de Eagle modifié par Dulbecco (DMEM) et de milieu nutritif F-12 de Ham est couramment rencontré dans la recherche en culture cellulaire. Pour compenser la perte de pouvoir tampon liée à la suppression du sérum, il est possible d'ajouter aux formulations du tampon HEPES (acide 4-(2-hydroxyéthyl)-1-pipérazineéthanesulfonique) à une concentration finale de 15 mM.
Application
Le milieu de Eagle modifié par Dulbecco/mélange nutritif F-12 de Ham :
- est entré dans la composition d'un milieu de culture pour cellules étoilées de foie humain primaires activées par culture[1].
- a été utilisé pour cultiver des biopsies de cartilage[2].
- peut servir à la propagation de Cowdria ruminantium.[3]
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Cet article | |||
|---|---|---|---|
| sterility sterile-filtered | sterility sterile-filtered | sterility - | sterility sterile-filtered |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form liquid | form liquid | form powder | form liquid |
| components glucose: 3.15 g/L | components glucose: 3.15 g/L | components sodium pyruvate: 0.055 g/L | components L-glutamine: no |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
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Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Dear, Can you tell me the composition of this medium, or at least the amount of L-Glutamine present in this media? Kind regards, Sarah
1 réponse-
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Is the glutamine that has already been added stable glutamine? How long is the shelf life?
1 réponse-
This product does not contain Alanyl-glutamine, which is considered a more stable form It has been supplemented with L-glutamine and will remain stable through out the shelf life of the product with proper storage and handling. Typical lots of D8437 are assigned an expiration date of 1 year from the date of release. Note that under culture conditions of 37 deg C the L-glutamine will gradually degrade. See the link below to review general stability information regarding L-glutamine.:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/195/924/t075.pdfAlanyl-glutamine is also available as a sterile medium supplement. Please see the link below for an example of one cell culture product option:
https://www.sigmaaldrich.com/product/sigma/g8541Utile ?
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Can Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham, be used under serum free conditions?
1 réponse-
When DME/F12 is used under serum free conditions, supplementation is necessary. DME/F12 can be used for growing the epithelial cell line TM4 (mouse testes) when supplemented with insulin, transferrin, EGF, FSH, somatomedin C, ovine growth hormone, and retinoic acid. Primary cultures of Sertoli cells can also be grown in the above supplemented media. Rat glial C6 cells have been grown in DME/F12 supplemented with transferrin, insulin, LH, and rat submaxillary gland extract. See: Jakoby, W.B., and Pastan, I.H., Cell Culture, Methods in Enzymology, 58, 103-106 (1979). (This book is listed as Product No. C0541).
Utile ?
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What is the storage temperature of Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham?
1 réponse-
We recommend storing Product No. D8437 at 2-8°C.
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What is Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham?
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This product is a 1:1 ratio mixture of Dulbecco’s Modified Eagle’s Medium (DME) high glucose and Nutrient Mixture F-12 Ham (F-12).
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the optimal carbon dioxide concentration needed for Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham?
1 réponse-
This is a 1:1 mixture of the DME and F-12. The optimal carbon dioxide required for DME is 10% and for F-12 is 5%. Since this medium is a mixture, the optimal carbon dioxide concentration is typically 5% to 8%.
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