D8062

DMEM/F-12

With ʟ-glutamine, sodium bicarbonate and sodium pyruvate, sterile liquid, suitable for cell culture

• Synonyme(s) : DME/F-12, mélange 1/1
• Numéro MDL: MFCD00217417
• Code UNSPSC : 12352207
• Nomenclature NACRES : NA.75

Propriétés

• product name: Milieu de Eagle modifié par Dulbecco (DMEM)/Mélange nutritif F-12 de Ham, With L-glutamine and sodium bicarbonate, without HEPES, liquid, sterile-filtered, suitable for cell culture
• Stérilité: sterile-filtered
• Forme: liquid
• Technique(s): cell culture | mammalian: suitable
• Impuretés: endotoxin, tested
• Composants: glucose: 3.15 g/L; NaHCO₃: yes; L-glutamine: yes; phenol red: yes; HEPES: no; sodium pyruvate: 0.055 g/L
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

Media are supplemented with a defined mixture of nutrients, growth factors, and hormones instead of serum. A 1:1 mixture of Dulbecco′s Modified Eagle′s medium (DME) and Ham′s F-12 nutrient mixture is commonly used for these defined studies. To compensate for the loss of buffering capacity caused by eliminating serum, a final concentration of 15 mM HEPES buffer can be added to the formulations. It allows the investigator to optimize the buffering system.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:

• to maintain human lung and colorectal cancer cell lines for the biological evaluation of the secondary metabolites
• to culture human kidney-2 (HK-2) cells and as a component of the assay medium for lysophosphatidylcholine (LPC) treatment
• to culture mouse squamous cell carcinoma (SCCVII) tumor cells

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable