D7656
Deoxyribonucleic acid, single stranded from salmon testes
For hybridization
Synonyme(s) :
single-stranded template DNA
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Numéro CAS:
Numéro MDL:
Code UNSPSC :
41106310
eCl@ss :
32160414
Nomenclature NACRES :
NA.52
Produits recommandés
Qualité
for molecular biology
Niveau de qualité
Pureté
9-11 mg/mL
Forme
solution
Concentration
10.0 ± 1.0 mg/mL in H2O
Conditions d'expédition
dry ice
Température de stockage
−20°C
Vous recherchez des produits similaires ? Visite Guide de comparaison des produits
Description générale
Deoxyribonucleic acid, single stranded from salmon testes is a ready-to-use solution of high quality, sonicated, single-stranded template DNA isolated from the testes of salmon.
Application
Deoxyribonucleic acid, single stranded from salmon testes has been used for/as:
- in situ hybridization histochemistry of rat brain, human kidney sections and renal biopsy tissues
- preparing standard curve of DNA
- prehybridization solution in southern and northern hybridizations
- dilution of the cDNA
- fluorescent in-situ hybridization (FISH)
- ChIP analysis
- a standard to analyse the degraded nucleosides of genomic DNA by high performance liquid chromatography
- a component of hybridization solution in FISH
- a control in DNA-DNA hybridization experiment between various bacterial species
- to precipitate probes in fluorescence in situ hybridization (FISH)
Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
Suitable for use as a blocking agent in Southern hybridizations.
Notes préparatoires
This DNA is phenol-chloroform extracted, ethanol precipitated, and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.
Autres remarques
DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Rune Thomsen et al.
PloS one, 8(8), e72110-e72110 (2013-08-31)
The intermediate filament network of astrocytes includes Glial fibrillary acidic protein (Gfap) as a major component. Gfap mRNA is alternatively spliced resulting in generation of different protein isoforms where Gfapα is the most predominant isoform. The Gfapδ isoform is expressed
XY aneuploidy rate in sperm of two ?minor? breeds of cattle (Bos taurus) by using dual color fluorescent in situ hybridization (FISH)
Pauciullo, Alfredo, et al
Theriogenology, 688-695 (2010)
Sydney Tang et al.
The Journal of clinical investigation, 111(4), 515-527 (2003-02-18)
Renal tubulointerstitial injury is characterized by inflammatory cell infiltrate; however, the stimuli for leukocyte recruitment are not fully understood. IL-8 is a potent chemokine produced by proximal tubular epithelial cells (PTECs). Whether nephrotic proteins stimulate tubular IL-8 expression remains unknown.
D Suzuki et al.
Diabetes, 44(10), 1233-1238 (1995-10-01)
Increased mesangial expansion is one of the most characteristic histological changes in diabetic nephropathy (DN). Although the pathogenesis of DN remains unclear, recent studies associate interleukin (IL) 6 with mesangial proliferative glomerulonephritis. To elucidate the expression and localization of IL-6
I Shibuya et al.
The Journal of physiology, 514 ( Pt 2), 351-367 (1998-12-16)
1. The expression, distribution and function of P2X purinoceptors in the supraoptic nucleus (SON) were investigated by reverse transcription-polymerase chain reaction (RT-PCR), in situ hybridization, and Ca2+-imaging and whole-cell patch-clamp techniques, respectively. 2. RT-PCR analysis of all seven known P2X
Articles
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique