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C7594

Sigma-Aldrich

Casein Blocking Buffer

for Northern and Southern blotting, powder blend

Synonyme(s) :

blocking buffer

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About This Item

Numéro CE :
232-555-1
Code UNSPSC :
12161700
Nomenclature NACRES :
NA.25

Qualité

for molecular biology

Forme

powder blend

Technique(s)

Southern blotting: suitable

pH

6.8-7.2(reconstituted per directions)

Solubilité

H2O: soluble, faintly yellow (dissolves in 5-10 minutes.)

Température de stockage

room temp

Catégories apparentées

Description générale

Casein is a milk-derived substance routinely used in blocking buffers for Western, Southern and Northern Blot procedures. Blocking agents are used to reduce non-specific binding of detection reagents to the membranes.

Application

Suitable as a blocking Buffer for Western, Southern and Northern Blot membranes. Blocked membranes can then be used in either chemiluminescent, colorimetric, or DIG detection assays.

Caractéristiques et avantages

  • Better blocking performance than dried milk or BSA
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent, colorimetric , and DIG detection assays

Notes préparatoires

Dissolve entire contents of bottle in 800 mL molecular biology grade water. Stir until well mixed, then add water to final volume of 1L. Store at 2-8°C for up to one week.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

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Solution tampon phosphate, 10× PBS for Western blots and IP

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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

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Tadatsura Koshika et al.
Immunology, 134(4), 386-397 (2011-11-03)
α1,3-Galactosyltransferase gene-knockout pigs transgenic for porcine cytotoxic T-lymphocyte antigen 4 immunoglobulin (pCTLA4-Ig) have been produced to reduce T-cell-mediated rejection following xenotransplantation. The level of soluble pCTLA4-Ig in their blood was greatly in excess of the therapeutic level in patients, rendering
Serapion Pyrpassopoulos et al.
Methods in molecular biology (Clifton, N.J.), 1046, 389-403 (2013-07-23)
Many proteins that reside in the cytoplasm bind directly to cell membranes and play roles in signaling, adhesion, metabolism, cell structure, and cell motility. Several of these membrane-binding proteins, especially cytoskeletal proteins, have mechanical functions that result in the transmission
Ochratoxin A in some French wines: application of a direct competitive ELISA based on an OTA-HRP conjugate.
Radoi, A., et al.
Analytical Letters, 42, 1187-1187 (2009)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Elizabeth R Sunderhaus et al.
Frontiers in neuroscience, 13, 1207-1207 (2019-11-30)
Mutations in patatin-like phospholipase domain-containing protein 6 (PNPLA6) have been linked with a number of inherited diseases with clinical symptoms that include spastic paraplegia, ataxia, and chorioretinal dystrophy. PNPLA6 is an evolutionary conserved protein whose ortholog in Drosophila is Swiss-Cheese
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Protocoles

Casein Blocking Buffer Protocol

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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