D5884
Dextranase from Penicillium sp.
lyophilized powder, 10-25 units/mg solid
Synonyme(s) :
1,6-α-D-Glucan 6-glucanohydrolase
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About This Item
Produits recommandés
Forme
lyophilized powder
Activité spécifique
10-25 units/mg solid
Température de stockage
2-8°C
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Application
Dextranase from Penicillium sp. has been used in a study to assess a new method of synthesizing biopolymeric affinity ligands. Dextranase from Penicillium sp. Has also been used in a study to investigate the cloning and sequencing of a dextranase-encoding cDNA from Penicillium minioluteum.
Dextranase from Sigma has been used for the hydrolysis of carbohydate polymers, during the study of polysaccharide synthesis by Phanerochaete chrysosporium. It has also been used in the synthesis of new enzymatically degradable thermo-responsive nanogels.
Actions biochimiques/physiologiques
An endodextranase that hydrolyzes α-(1,6)-glucosidic linkages in dextran. Dextrans are undesirable compounds synthesized from sucrose by microbial contaminants during sugar production. They increase viscosity of the flow and decrease industrial recovery. Dextranase has been used for hydrolyzing dextran at sugar mills in order to improve efficiency of sugar production.
Qualité
Crude
Définition de l'unité
One unit will liberate 1.0 μmole of isomaltose (measured as maltose) per min at pH 6.0 at 37 °C, using dextran as substrate.
Autres remarques
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Les clients ont également consulté
G S Chaga et al.
Biotechnology and applied biochemistry, 26 ( Pt 1), 7-14 (1997-08-01)
(1) A new concept for producing soluble polymeric affinity ligands is proposed and exemplified. By solid-phase synthesis, an insoluble hydrophilic polymer is converted into an affinity gel. The gel is hydrolytically degraded to water-soluble affinity polymeric ligands which are recovered
H Roca et al.
Yeast (Chichester, England), 12(12), 1187-1200 (1996-09-30)
The DEX gene encoding an extracellular dextranase was isolated from the genomic DNA library of Penicillium minioluteum by hybridization using the dextranase cDNA as a probe. Comparison of the gene and cDNA sequences revealed that the DEX gene does not
Synthesis of new enzymatically degradable thermo-responsive nanogels.
Aguirre, G., Ramos, J., & Forcada, J
Soft Matter, 9(1), 261-270 (2013)
Polysaccharide synthesis by Phanerochaete chrysosporium during degradation of kraft lignin
Leisola M, et al.
Europ. J. Appl. Microbiol. Biotechnol., 15(3), 180-184 (1982)
B Garcia et al.
FEMS microbiology letters, 143(2-3), 175-183 (1996-10-01)
A cDNA from Penicillium minioluteum HI-4 encoding a dextranase (1,6-alpha-glucan hydrolase, EC 3.2.1.11) was isolated and characterized. cDNA clones corresponding to genes expressed in dextran-induced cultures were identified by differential hybridization. Southern hybridization and restriction mapping analysis of selected clones
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