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D0547

Sigma-Aldrich

Milieu de Eagle modifié par Dulbecco (DMEM)/Mélange nutritif F-12 de Ham

With L-glutamine and trace elements, without HEPES and sodium bicarbonate, powder, suitable for cell culture

Synonyme(s) :

DME/F-12, mélange 1/1

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About This Item

Numéro MDL:
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.71

Niveau de qualité

Forme

powder

Technique(s)

cell culture | mammalian: suitable

Composants

NaHCO3: no
sodium pyruvate: 0.11 g/L
HEPES: no
phenol red: yes
glucose: 3.15 g/L
L-glutamine: yes

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Description générale

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is a 1:1 mixture.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:

  • to culture cerebral cortices from mice embryos
  • as a component of the human corneal epithelium (HCE) media for culturing human limbal explants
  • to culture human corneal epithelial cell lines

Autres remarques

Trace elements include ammonium molybdate, ammonium metavanadate, stannous chloride, sodium selenite, magnesium sulfate, nickel chloride, and sodium metasilicate

Quantité

Formulated to contain 12.0 grams of powder per liter of medium.

Reconstitution

Supplement with 1.2 g/L sodium bicarbonate

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Optimizing the role of limbal explant size and source in determining the outcomes of limbal transplantation: An in vitro study
Abhinav Reddy K, et al.
PLoS ONE (2017)
LPA2 promotes neuronal differentiation and neurite formation in neocortical development
Mizuki U, et al.
Biochemical and Biophysical Research Communications, 89-94 (2022)
Ceyda Acilan et al.
Biochimica et biophysica acta, 1861(2), 218-234 (2016-10-25)
To overcome the hurdles of cisplatin, majorly its toxicity and resistance, there has been extensive search for alternative anti-cancer metal-based compounds. Here, three Cu(II)-complexes, Cu(Sal-Gly)(phen), Cu(Sal-Gly)(pheamine), Cu(Sal-Gly)(phepoxy) are characterized for their interaction with DNA, cytotoxicity and mechanism of action. The
Christo J Botha et al.
The Onderstepoort journal of veterinary research, 87(1), e1-e4 (2020-04-04)
Diplodiosis is an important neuromycotoxicosis of ruminants in South Africa when grazing on harvested maize fields in winter. It is believed to be caused by mycotoxin(s) synthesised by Stenocarpella (Diplodia) maydis. Although several metabolites have been isolated from S. maydis
Selma M Soyal et al.
Molecular neurobiology, 57(2), 752-764 (2019-09-01)
PPARGC1A encodes a transcriptional co-activator also termed peroxisome proliferator-activated receptor (PPAR) gamma coactivator 1-alpha (PGC-1α) which orchestrates multiple transcriptional programs. We have recently identified CNS-specific transcripts that are initiated far upstream of the reference gene (RG) promoter. The regulation of

Articles

Nutrient Mixture F-12 Ham Formulation

Protocoles

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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