CS1030

Chitinase Assay Kit, Fluorimetric

sufficient for 200 multiwell tests

• Synonyme(s) : Chitinase Detection Kit
• Code UNSPSC : 12161503
• Nomenclature NACRES : NA.84

Propriétés

• Utilisation: sufficient for 200 multiwell tests
• Niveau de qualité: 200
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... CHIT1(1118)

Description

Description générale

The kit assay is based on the enzymatic hydrolysis of chitinase substrates. This enzymatic hydrolysis releases 4-methylumbelliferone (4MU), which upon ionization in basic pH, can be measured fluorimetrically at an excitation wavelength of 360 nm and an emission wavelength of 450 nm. The use of fluorimetric substrates provides a very sensitive detection system.

Application

The Chitinase Assay Kit provides all the reagents required for efficient and sensitive detection of chitinase activity in fungal and bacterial growth media, macrophage lysates, and purified enzyme preparations. In addition, the kit provides three different substrates for the detection of the various types of the chitinolytic activity:

• 4-Methylumbelliferyl N,N′-diacetyl-β-D-chitobioside - substrate suitable for exochitinase activity detection (chitobiosidase activity)
• 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide - substrate suitable for exochitinase activity detection (β-N-acetylglucosaminidase activity)[1]
• 4-Methylumbelliferyl β-D-N,N′,N′′-triacetylchitotriose - substrate suitable for endochitinase activity detection

Actions biochimiques/physiologiques

Chitinase catalyzes the hydrolytic cleavage of the β-1→4-glycoside bond present in biopolymers of N-acetylglucosamine, primarily in chitin. Chitinases are widely distributed in living organisms and are found in fungi, bacteria, parasites, plants, and animals. They are classified in families based on amino acid sequence similarities.
The chitinolytic enzymes are also categorized based on their enzymatic action on chitin substrates. Endochitinases are defined as the enzymes catalyzing the random cleavage at internal points in the chitin chain. Exochitinases catalyze the progressive release of acetylchitobiose or N-acetylglucosamine from the non-reducing end of chitin, and are referred to as chitobiosidase and β-N-acetylglucosaminidase, respectively.
Chitinases perform different functions in different organisms. In bacteria, they are mainly involved in nutritional processes. In yeast and various fungi, these enzymes participate in morphogenesis. In animals and plants, chitinases primarily play a role in the defense of the organism against pathogen attack.

Adéquation

The kit was tested and found suitable for Trichoderma viride and Streptomyces griseus, along with Hela, Jurkat, CHO, NIH-3T3, U-837 mammalian cell lines, human macropages, rat lung, kidney, liver, and brain tissue.

Notes préparatoires

Use ultrapure water for preparation of reagents.

Composants de kit seuls

• Assay Buffer 25 mL
• 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide 5 mg
• 4-Methylumbelliferyl β-D-N,N′-diacetylchitobioside hydrate 5 mg
• 4-Methylumbelliferyl β-D-N,N′,N′′-triacetylchitotriose 5 mg
• Chitinase from Trichoderma viride 1 mg
• 4-Methylumbelliferone Standard Solution, 50 mg/mL 1 mL
• Sodium Carbonate 2 g
Dimethyl Sulfoxide 1 mL

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Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H319,H334
• Conseils de prudence: P261 - P264 - P280 - P284 - P305 + P351 + P338 - P337 + P313
• Classification des risques: Eye Irrit. 2 - Resp. Sens. 1
• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable