EIII

86072804, Human x Mouse Hybridoma hybrid, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• product name: EIII, 86072804
• Source biologique: Human x Mouse Hybridoma hybrid
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: pJ3.11, p79a, CFTR DNA markers
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human x mouse hybrid

Description de la lignée cellulaire

This cell line may contain a sub-chromosomal fragment of human chromosome 17 bearing markers thought at the time to flank the cytotic fibrosis gene. The human DNA was donated from the human osteosarcoma cell line MNNG-HOS.

Application

Somatic cell hybrid mapping

Milieu de culture

DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 37°C; 5% CO₂.

Autres remarques

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.