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C8274

Sigma-Aldrich

Cytohelicase from Helix pomatia

lyophilized powder

Synonyme(s) :

Snail gut enzyme

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About This Item

Numéro CAS:
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Forme

lyophilized powder

Composition

Protein, 50-80% Lowry

Température de stockage

2-8°C

Application

Cytohelicase from Helix pomatia has been used:
  • in the enzyme solution for preparing the cell nuclei for immuno fluorescent in situ hybridization (immunoFISH)
  • in the enzyme mix to analyze meiotic cells at diakinesis and later stages in anthers
  • in the enzyme mix for the analysis of mitosis in anthers

Cytohelicase from Helix pomatia has been used to study the isolation of yeast protoplasts using various preparations of the hepato-pancreatic juice of Helix pomatia.
The enzyme from Sigma has been used for cytohelicase assay. The enzyme assay was used while studying the effects of MNN10 gene-disruption on protein secretion in Kluyveromyces lactis and Saccharomyces cerevisiae cultures.

Actions biochimiques/physiologiques

Cytohelicase from Helix pomatia is used in the preparation of synaptonemal complex spreading techniques along with bovine serum albumin. It plays an important role in the digestion of cell wall. Cytohelicase interacts with chromatin and improves the adhesion of synaptonemal complexes to the hydrophobic plastic film.

Remarque sur l'analyse

Contains glucanase/laminarinase activity

Pictogrammes

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Conseils de prudence

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Consulter la Bibliothèque de documents

Sònia Garcia et al.
BMC plant biology, 12, 95-95 (2012-06-22)
In plants, the 5 S rRNA genes usually occur as separate tandems (S-type arrangement) or, less commonly, linked to 35 S rDNA units (L-type). The activity of linked genes remains unknown so far. We studied the homogeneity and expression of
Simon Amiard et al.
PloS one, 9(1), e86220-e86220 (2014-01-28)
In striking contrast to animals, plants are able to develop and reproduce in the presence of significant levels of genome damage. This is seen clearly in both the viability of plants carrying knockouts for key recombination and DNA repair genes
Danguole Bartkeviciūte et al.
FEMS yeast research, 4(8), 833-840 (2004-09-29)
Screening for genes affecting super-secreting phenotype of the over-secreting mutant of Kluyveromyces lactis resulted in isolation of the gene named KlMNN10, sharing high homology with Saccharomyces cerevisiae MNN10. The disruption of the KlMNN10 in Kluyveromyces lactis, as well as of
Mikhail G Divashuk et al.
PloS one, 9(1), e85118-e85118 (2014-01-28)
Hemp (Cannabis sativa L.) was karyotyped using by DAPI/C-banding staining to provide chromosome measurements, and by fluorescence in situ hybridization with probes for 45 rDNA (pTa71), 5S rDNA (pCT4.2), a subtelomeric repeat (CS-1) and the Arabidopsis telomere probes. The karyotype
Ribosomal DNA locus evolution in Nemesia: transposition rather than structural rearrangement as the key mechanism?
Datson PM and Murray BG
Chromosome Research, 14(8), 845-857 (2006)

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