AMPD1
DNase I
Amplification Grade
Synonyme(s) :
Deoxyribonuclease I
About This Item
Produits recommandés
Forme
liquid
Concentration
1 unit/μL
Technique(s)
RT-PCR: suitable
Couleur
colorless
Conditions d'expédition
wet ice
Température de stockage
−20°C
Catégories apparentées
Description générale
DNase I digests double- and single-stranded DNA into oligo- and mononucleotides. Using the Reaction Buffer provided, DNA is removed from RNA preparations in a 15 minute digestion at room temperature. The DNase I is then inactivated by heating with the Stop Solution. Heating also denatures hairpins in the RNA, so the RNA can be used directly in reverse transcription.
No current RNA isolation procedure removes 100% of the DNA. Many commercial DNase I formulations are contaminated with residual RNases. This RNase contamination can destroy or degrade valuable RNA samples prior to reverse transcription. Laboratory comparisons have shown that Sigma′s Amplification Grade DNase I demonstrates lower RNase activity than that from several leading molecular biology product suppliers.
Application
- for the digestion of DNA during isolation and purification of RNA. The purified RNA can be used for the synthesis of cDNA using RNA reverse transcriptase.
- to hydrolyze extracellular matrix (ECM) components and enhance photosensitizer penetration into the biofilm to determine the efficacy of antimicrobial photodynamic therapy (aPDT) on Candida albicans biofilms
- to remove contaminating DNA from total RNA extracted from cattle blood samples
Caractéristiques et avantages
- Suitable for the elimination of DNA from RNA
- Minimal RNase activity available
- Optimized 10× reaction buffer and Stop Solution for complete inactivation of DNase I
Adéquation
Définition de l'unité
Informations légales
Inhibiteur
Produit(s) apparenté(s)
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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