Phosphate Buffer Saline (PBS) and Tris Buffer Saline (TBS) are concentrated wash solutions designed for rinsing reagents from tissue slides and preserving tissue sections between reagent applications. PBS is recommended with any detection system, except those containing alkaline phosphatase. Conversely, TBS is recommended when alkaline phosphatase is present to avoid excessive phosphate accumulation and consequent reduction in the rate of reaction. Adding Tween® 20 to both PBS and TBS may help reduce background and produce a cleaner immunohistochemical stain overall. The most significant factor is the intention for use to visualize the stain, with what chromogen and detection kit. For HRP or Alk Phos on the breast panel, TBS can be used for both. For diluent, as long as products are stored properly (2 to 8C), the diluent will help stabilize diluted antibodies, and the same applies for use with HRP or Alk Phos detection for TBS to be run on either, while PBS works best with HRP. This allows for a longer expiration date and aligns with the antibody expiration date.
935B
TBS IHC Wash Buffer + TWEEN® 20
20X TBS, 1% Tween® 20
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| technique(s) immunohistochemistry: suitable | technique(s) immunohistochemistry: suitable | technique(s) - | technique(s) - |
| Quality Level 500, 600 | Quality Level 500, 600 | Quality Level 100 | Quality Level 100 |
| form buffered aqueous solution | form buffered aqueous solution | form liquid | form tablet |
| storage temp. 2-8°C | storage temp. - | storage temp. - | storage temp. 15-25°C |
| description [For In Vitro Diagnostic Use in Select Regions] | description [For In Vitro Diagnostic Use in Select Regions] | description - | description - |
| shipped in wet ice | shipped in ambient | shipped in - | shipped in ambient |
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WGK 1
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Is there a recommended or preferable wash buffer (TBS or PBS) for a breast panel in Autostainer? Moreover, please confirm how long one can wait once the dilution of the concentrated product is done? Both TBS and PBS in this case.
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