N1636

β-Nicotinamide-adénine-dinucléotide hydrate

purified by column chromatography, ≥99%

• Synonyme(s) : β-DPN, β-NAD, Coenzyme 1, Cozymase, DPN, Diphosphopyridine nucléotide, NAD, Nadide
• Formule empirique (notation de Hill) : C₂₁H₂₇N₇O₁₄P₂ · xH₂O
• Numéro CAS: 53-84-9
• Poids moléculaire : 663.43 (anhydrous basis)
• Numéro CE : 200-184-4
• Numéro MDL: MFCD00036253
• Code UNSPSC : 41106305
• eCl@ss : 39200202
• ID de substance PubChem : 24897510
• Nomenclature NACRES : NA.55

Propriétés

• Niveau de qualité: 200
• Essai: ≥99%
• Forme: crystalline powder
• Couleur: white
• Température de stockage: −20°C
• Chaîne SMILES: NC1=NC=NC2=C1N=CN2.O[C@@H]3[C@@H](COP(O)(OP(OC[C@@H](O4)[C@@H](O)[C@@H](O)[C@H]4[N+]5=CC=CC(C(N)=O)=C5)([O-])=O)=O)OC[C@@H]3O
• InChI: 1S/C21H27N7O14P2/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33/h1-4,7-8,10-11,13-16,20-21,29-32H,5-6H2,(H5-,22,23,24,25,33,34,35,36,37)/t10-,11-,13-,14-,15-,16-,20-,21-/m1/s1
• Clé InChI: BAWFJGJZGIEFAR-NNYOXOHSSA-N

Description

Description générale

β-Nicotinamide adenine dinucleotide (NAD) is an electron carrier[1] and a cofactor. It is ubiquitously found in the living system. NAD⁺ contains an adenylic acid and a nicotinamide-5′-ribonucleotide group linked together by a pyrophosphate moiety. In NAD⁺ complexes, the enzyme-cofactor interactions are highly conserved.[2]

Application

β-Nicotinamide adenine dinucleotide hydrate has been used:

• to measure adenosine triphosphate (ATP) synthesis rates (kinetics measurements)[3]
• in the reaction buffer for pyruvate kinase (PK) activity assay[4]
• as a component of master mix for a high throughput screening[5]

Actions biochimiques/physiologiques

Accepteur d'électrons

β-Nicotinamide adenine dinucleotide (NAD) cycles between the oxidized (NAD⁺⁾ and reduced (NADH) forms to maintain a redox balance necessary for continued cell growth.[6] It significantly participates in enzyme-catalyzed oxido-reduction processes[2] and many genetic processes. It is also involved in microbial catabolism.[6]

Conditionnement

Produit conditionné en fonction du poids solide.

Autres remarques

Crystalline NAD contains less than 0.1% (w/w) residual organic solvent, is less hygroscopic and electrostatic, and is more stable at ambient temperatures than amorphous NAD.

Il s′agit de la forme courante du NAD.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)