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85463

Millipore

Simmons Citrate Agar

NutriSelect® Basic, suitable for microbiology

Synonyme(s) :

Citrate Utilization Test

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About This Item

Code UNSPSC :
41171606
Nomenclature NACRES :
NA.74

Stérilité

non-sterile

Niveau de qualité

Gamme de produits

BioChemika

Forme

powder

Durée de conservation

limited shelf life, expiry date on the label

Composition

agar, 15 g/L
ammonium dihydrogen phosphate, 0.2 g/L
bromothymol blue, 0.08 g/L
disodium ammonium phosphate, 0.8 g/L
magnesium sulfate heptahydrate, 0.2 g/L
sodium chloride, 5 g/L
trisodium citrate, 2 g/L

Fabricant/nom de marque

NutriSelect® Basic

Technique(s)

microbe id | utilization test: suitable
microbiological culture: suitable

pH final

~7.0

Application(s)

agriculture
clinical testing
environmental
food and beverages
water monitoring

microbiology

Adéquation

nonselective and differential for Escherichia coli
nonselective and differential for Salmonella spp.
nonselective and differential for coliforms
nonselective and differential for molds (General Media)
nonselective and differential for yeasts (General Media)

Description générale

Simmons Citrate Agar uses citric acid as the carbon source. It is used to differentiate Salmonella groups from each other. It does not allow the growth of Shigella or coliforms.

Application

Simmons Citrate Agar can be used for differentiating between fecal coli and members of the aerogenes group based on citrate utilization. It can also be used for the identification of certain fungi and fungi imperfecti. The agar has been used to perform a citrate test.

Notes préparatoires

Suspend 23.3 g in 1 litre distilled water and heat to boiling to dissolve. Distribute in tubes or flasks and sterilize by autoclaving at 121°C for 15 minutes. Allow tubes to cool as slants.

Autres remarques

Selective recovery of Klebsiella pneumoniae and Klebsiella oxytoca from human feces

Note de bas de page

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informations légales

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Elmer W. Koneman
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International Journal of Systematic and Evolutionary Microbiology (2006)
R.K. Dart
Microbiology for the Analytical Chemist (1996)
E Van Kregten et al.
Journal of clinical microbiology, 20(5), 936-941 (1984-11-01)
A culture medium was developed which selectively favored the growth of Klebsiella pneumoniae and Klebsiella oxytoca in Escherichia coli-rich fecal cultures, without the use of antibiotics. The discriminative capacity of this medium was based on the presence of only two

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