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11966006001

Roche

TUNEL Dilution Buffer

pH 7.2-7.4 (20 °C), suitable for detection, pkg of 2 × 10 mL

Synonyme(s) :

TUNEL, transferase dUTP nick end labeling

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About This Item

Code UNSPSC :
41116100

Conditionnement

pkg of 2 × 10 mL

Fabricant/nom de marque

Roche

pH

7.2-7.4 (20 °C)

Application(s)

detection

Température de stockage

2-8°C

Description générale

The TUNEL dilution buffer can be used in combination with the in-situ cell death detection kits. It can be used to dilute terminal deoxynucleotidyl transferase (TdT) for the TUNEL reaction in special applications and also to label DNA strand breaks for detecting and quantitating apoptotic cell death at the single-cell level in cells and tissues.

Application

TUNEL Dilution Buffer has been used:
  • to dilute terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) enzyme (TdT) in preparing TUNEL reaction mixture for TUNEL critical commercial assays
  • to quantify apoptosis in rat kidney
  • to dilute the terminal deoxynucleotidyl transferase enzyme solution to identify DNA fragmentation in oligodendrocytes in vitro
  • for TUNEL assays

TUNEL dilution buffer has been used in terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays.

Notes préparatoires

Working concentration: Working concentration of conjugate depends on application and substrate.


The following concentrations should be taken as a guideline:
  • ELISA: 100 mU/ml
  • Western blot: 100mU/ml


Storage conditions (working solution): Dilutions of TdT should be prepared immediately before use and should not be stored.
Keep TUNEL reaction mixture on ice until use.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Pictogrammes

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Aquatic Chronic 3 - Carc. 1B Inhalation

Code de la classe de stockage

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash


Listes réglementaires

Les listes réglementaires sont principalement fournies pour les produits chimiques. Seules des informations limitées peuvent être fournies ici pour les produits non chimiques. L'absence d'indication signifie qu'aucun des composants n'est répertorié. Il incombe à l'utilisateur de s'assurer de l'utilisation sûre et légale du produit.

EU REACH Annex XVII (Restriction List)


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