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11767305001

Roche

TUNEL Enzyme

from calf thymus recombinant in E. coli

Synonyme(s) :

transferase dUTP nick end labeling, tunel

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About This Item

Code UNSPSC :
41105600

Produit recombinant

expressed in E. coli

Niveau de qualité

100

Forme

solution

Utilisation

sufficient for 20 tests

Conditionnement

pkg of 2 × 50 μL

Fabricant/nom de marque

Roche

Température de stockage

−20°C

Catégories apparentées

Description générale

TUNEL enzyme solution, containing terminal deoxynucleotidyl transferase from calf thymus recombinant in E. coli.
One of the two components required for performing the TUNEL reaction to detect apoptosis in situ.

Application

The enzyme solution contains terminal deoxynucleotidyl transferase from calf thymus, recombinant in E. coli, needed to perform the TUNEL reaction for detecting apoptosis in situ.
The TUNEL reaction mixture is used to label DNA strand breaks for detecting and quantitating apoptotic cell death at the single-cell level in cells and tissues.

Forme physique

Terminal deoxynucleotidyl transferase (TdT) in an optimized concentration, dissolved in reaction buffer. Ready-to-use with TUNEL Label.

Notes préparatoires

Working solution: TUNEL Enzyme is used in combination with TUNEL Label to prepare the TUNEL reaction mixture.
To produce a TUNEL solution sufficient for one test:
  • Mix 5μl TUNEL Enzyme with 45μl TUNEL Label prior to use.
  • For negative control use 50μl/test TUNEL Label only.

Storage conditions (working solution): Note: The TUNEL reaction mixture (45μl TUNEL Label with 5μl TUNEL Enzyme for 1 test) should be prepared immediately before use and should not be stored. Keep TUNEL reaction mixture on ice until use.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

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