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MABT892

Sigma-Aldrich

Anti-SUN1 Antibody, clone 12.10F

clone 12.10F, from mouse

Synonyme(s) :

SUN domain-containing protein 1, Protein unc-84 homolog A, Sad1/unc-84 protein-like 1

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

12.10F, monoclonal

Espèces réactives

mouse, rat

Ne doit pas réagir avec

human

Technique(s)

immunocytochemistry: suitable
western blot: suitable

Isotype

IgG2aκ

Numéro d'accès NCBI

NP_077771

Numéro d'accès UniProt

Q9D666

Conditions d'expédition

ambient

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

mouse ... Sun1(77053)

Description générale

SUN domain-containing protein 1 (UniProt Q9D666; also known as Protein unc-84 homolog A, Sad1/unc-84 protein-like 1) is encoded by the Sun1 (also known as Unc84a) gene (Gene ID 77053) in murine species. SUN1 is a component of the SUN-protein-containing complexes known as LINC (linker of nucleoskeleton and cytoskeleton), which link the nucleoskeleton and cytoskeleton structures together in a scaffold like fashion. SUN1 is required for interkinetic nuclear migration and nucleokinesis as well as nucleus-centrosome coupling during radial neuronal migration and glial migration in the cortex. SUN1 also is important during meiosis and is required for proper gametogenesis. It also participates in anchoring chromosome movement in the prophase of meiosis and in selective gene expression of coding and non-coding RNAs required for gametogenesis. Additionally, it has a vital role in the distribution of nuclear pore complexes on the nuclear membrane. Interestingly, LINC complexes are also critical for vital functions such as hearing.

Spécificité

Clone 12.10F was raised against a fusion construct composed of murine SUN1 exon 6-, 8-, and 10-encoded regions that are present in murine spliced isoforms 1, 3, and 4 reported by UniProt (Q9D666). The epitope location among these three regions has not been mapped and reactivity toward murine spliced isoform 3 is expected only if the epitope resides within exon 1- or 4-encoded region.

Immunogène

GST-tagged recombinant fusion protein composed of murine SUN1 regions encoded by exons 6, 8, and 10.

Application

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected SUN1 nuclear immunoreactivity in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized C2C12 murine myoblasts.

Immunocytochemistry Analysis: A representative lot immunostained nucleus of C2C12 murine myoblasts (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR, Singapore).

Western Blotting Analysis: A representative lot detected greatly reduced SUN1 isoforms target bands in lysate from SUN1 siRNA-treated C2C12 murine myoblasts (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR, Singapore).
Research Category
Cell Structure
This mouse monoclonal Anti-SUN1 Antibody, clone 12.10F, Cat. No. MABT892 is validated for use in Immunocytochemistry and Western Blotting for the detection of SUN1.

Qualité

Evaluated by Western Blotting in C2C12 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SUN1 in 10 µg of C2C12 murine myoblast lysate.

Description de la cible

~102 kDa oberved. 102.0/88.20/97.92/94.51 kDa (mouse isoform 1/2/3/4) calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified
Protein G purified.
Purified mous IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Mark A Smith et al.
Heliyon, 8(12), e12147-e12147 (2023-01-10)
Formation of robust actomyosin stress fibers (SF) in response to cell stretch plays a key role in the transfer of information from the cytoplasm into the nucleus. Actin/LINC/Lamin (ALL) nuclear lines provide mechanical linkage between the actin cytoskeleton and the
Joana T Lima et al.
Life science alliance, 7(4) (2024-01-17)
Accurate centrosome separation and positioning during early mitosis relies on force-generating mechanisms regulated by a combination of extracellular, cytoplasmic, and nuclear cues. The identity of the nuclear cues involved in this process remains largely unknown. Here, we investigate how the
Disulfide bond in SUN2 regulates dynamic remodeling of LINC complexes at the nuclear envelope.
Sharma, et al.
Life science alliance, 6 (2023)

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