MABT880
Anti-SUN2 Antibody, clone 3.1E
clone 3.1E, from mouse
Synonyme(s) :
SUN domain-containing protein 2, Protein unc-84 homolog B, Rab5-interacting protein, Rab5IP, Sad1/unc-84 protein-like 2
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
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Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
3.1E, monoclonal
Espèces réactives
human, rat, mouse
Technique(s)
immunocytochemistry: suitable
western blot: suitable
Isotype
IgG1κ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
ambient
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... SUN2(25777)
Description générale
SUN domain-containing protein 2 (UniProt Q9UH99; also known as Protein unc-84 homolog B, Rab5-interacting protein, Rab5IP, Sad1/unc-84 protein-like 2) is encoded by the SUN2 (also known as FRIGG, KIAA0668, RAB5IP, UNC84B) gene (Gene ID 25777) in human. It is a single-pass nuclear envelope transmembrane protein that is highly expressed in heart, lung, and muscle. It has a predicted transmembrane domain and a C-terminal region with similarity to the S. pombe spindle pole body protein Sad1. It may also facilitate a nuclear-centrosomal interaction required for nuclear migration and anchorage. It anchors chromosome movement in the prophase of meiosis and is required for telomere attachment to nuclear envelope and gametogenesis. SUN2 protein may also function on endocytic vesicles as a receptor for RAB5-GDP and participate in the activation of RAB5. Slight overexpression of SUN2 protein is seen in heart tissues form patients with congenital heart defects.
Spécificité
Target specificity of clone 3.1E was verified by immunocytochemistry and Western blotting analyses using fibroblasts and heart tissue samples from Sun2-knockout mice.
Immunogène
GST-tagged recombinant human SUN2 N-terminal LMNA-binding region fragment.
Application
Research Category
Cell Structure
Cell Structure
This mouse monoclonal Anti-SUN2 Antibody, clone 3.1E, Cat. No. MABT880, is validated for use in Immunocytochemistry and Western Blotting for the detection of SUN2.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected SUN2 in 10 µg of HepG2 cell lysate.
Immunocytochemistry Analysis: A representative lot immunostained nucleus of fibroblasts from Sun2+/-, but not Sun2-/- mice (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).
Western Blotting Analysis: A representative lot detected SUN2 in heart tissue lysate from Sun2+/-, but not Sun2-/- mice (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).
Immunofluorescence Analysis (IF): A representative lot detected mouse testis tissue (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).
Immunofluorescence Analysis (IF): A representative lot tested on different mouse tissues (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).
Immunofluorescence Analysis (IF): A representative lot detected immortalized fibroblasts derived from SUN2 +/+, SUN2 -/- , Sun1 -/- double knockout Sun2 -/- Sun1 -/- mouse pups (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).
Immunocytochemistry Analysis: A representative lot immunostained nucleus of fibroblasts from Sun2+/-, but not Sun2-/- mice (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).
Western Blotting Analysis: A representative lot detected SUN2 in heart tissue lysate from Sun2+/-, but not Sun2-/- mice (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).
Immunofluorescence Analysis (IF): A representative lot detected mouse testis tissue (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).
Immunofluorescence Analysis (IF): A representative lot tested on different mouse tissues (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).
Immunofluorescence Analysis (IF): A representative lot detected immortalized fibroblasts derived from SUN2 +/+, SUN2 -/- , Sun1 -/- double knockout Sun2 -/- Sun1 -/- mouse pups (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).
Qualité
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected SUN2 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected SUN2 in 10 µg of HeLa cell lysate.
Description de la cible
~80 kDa observed. 80.31/82.50/79.61 kDa (isoform 1/2/3) calculated. Uncharacterized bands may be observed in some lysate(s).
Forme physique
Format: Purified
Protein G purified.
Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Certificats d'analyse (COA)
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Joana T Lima et al.
Life science alliance, 7(4) (2024-01-17)
Accurate centrosome separation and positioning during early mitosis relies on force-generating mechanisms regulated by a combination of extracellular, cytoplasmic, and nuclear cues. The identity of the nuclear cues involved in this process remains largely unknown. Here, we investigate how the
Fangfang Yang et al.
Frontiers in bioengineering and biotechnology, 8, 647-647 (2020-07-17)
Atherosclerotic plaque preferentially develops in arterial curvatures and branching regions, where endothelial cells constantly experience disturbed blood flow. By contrast, the straight arteries are generally protected from plaque formation due to exposure of endothelial cells to vaso-protective laminar blood flow.
Victoria Bryson et al.
The Journal of clinical investigation, 134(7) (2024-02-01)
Stromal interaction molecule 1 (STIM1) is a Ca2+ sensor located in the sarcoplasmic reticulum (SR) of skeletal muscle, where it is best known for its role in store-operated Ca2+ entry (SOCE). Genetic syndromes resulting from STIM1 mutations are recognized as
Andres Ramirez-Martinez et al.
Nature communications, 12(1), 690-690 (2021-01-31)
Lamins and transmembrane proteins within the nuclear envelope regulate nuclear structure and chromatin organization. Nuclear envelope transmembrane protein 39 (Net39) is a muscle nuclear envelope protein whose functions in vivo have not been explored. We show that mice lacking Net39 succumb
Disulfide bond in SUN2 regulates dynamic remodeling of LINC complexes at the nuclear envelope.
Sharma, et al.
Life science alliance, 6 (2023)
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