ECM220
QCM Laminin Migration Assay (24-well, colorimetric)
About This Item
Produits recommandés
Fabricant/nom de marque
Chemicon®
QCM
Niveau de qualité
Technique(s)
cell based assay: suitable
Méthode de détection
colorimetric
fluorometric
Description générale
Cell migration may be evaluated using several different methods; the most widely accepted being the Boyden Chamber assay. The Boyden Chamber system uses a two-chamber plate model in which a porous membrane provides an interface between two chambers. Cells are seeded in the upper chamber and chemoattractants placed in the lower chamber. Cells in the upper chamber migrate toward the chemoattractants by passing through the porous membrane to the lower chamber. Migratory cells are stained and quantified. Most cells are sized from 30 to 50 μm can migrate through 3 to 10 μm pore.
Application
Cell Structure
Laminin protein is coated on the bottom of each chamber′s porous membrane as the driver for cell migration. A similarly BSA-coated control chamber(s) provides an appropriate migration control. Cells that migrate toward laminin are stained using crystal violet, a nucleic dye, extracted from the membrane surface, and quantified on a microplate reader using spectrophotometry.
Each kit provides sufficient materials for the evaluation of 24 samples.
Conditionnement
Composants
1. 24-well Cell Migration Plate Assembly - 5 μm: (Part No. 2005708) Two 24-well plates, each containing 12- 5 μm pore inserts per plate.
2. BSA 30% solution: (Part No. CS203352) One vial containing 0.5 mL of a 30% solution.
3. Cell Stain Solution*: (Part No. 90144) One bottle.
4. Extraction Buffer: (Part No. 90145) One bottle.
5. 24-well Stain Receiver Plate: (Part No. PIMWS2450) One each.
6. 96-Well Stain Quantitation Plate: (Part No. 2005870) One each.
7. Swabs: 50 ea
8. Forceps: 1 pair
*Caution: Cell Stain Solution contains a small amount of crystal violet, which is toxic if swallowed or inhaled, and may cause irritation to the eyes, respiratory system, and skin. Handle with caution.
ECM220-2
1. Laminin: (Part No. CS202685) Two vials each containing 60 μg in solution.
Stockage et stabilité
- ECM220-1 kit components should be stored at 2° to 8°C. DO NOT FREEZE.
- ECM220-2 component must be stored at -20°C until needed.
Informations légales
Clause de non-responsabilité
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Flam. Liq. 2
Code de la classe de stockage
3 - Flammable liquids
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