APT131
Caspase 3 Colorimetric Activity Assay Kit, DEVD
Caspase-3 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence DEVD.
Synonyme(s) :
CPP32
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12161503
eCl@ss :
32161000
Nomenclature NACRES :
NA.54
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Niveau de qualité
Réactivité de l'espèce (prédite par homologie)
mammals
Fabricant/nom de marque
Chemicon®
Technique(s)
activity assay: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Méthode de détection
colorimetric
Conditions d'expédition
dry ice
Informations sur le gène
human ... CASP3(836)
Description générale
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon′s Caspase-3 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3 activity.
Application
Caspase-3 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence DEVD.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Composants
5X Cell Lysis Buffer (Part No. 90065): 5 mL
5X Assay Buffer (Part No. 90066): 10 mL
Caspase-3 Substrate (Ac-DEVD-pNA) (Part No. 90079): 250 μL of 3 mg/mL solution
Caspase-3 Inhibitor (Ac-DEVD-CHO) (Part No. 90067): 50 μL of 100 μM (0.05 mg/mL) in DMSO
pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO
5X Assay Buffer (Part No. 90066): 10 mL
Caspase-3 Substrate (Ac-DEVD-pNA) (Part No. 90079): 250 μL of 3 mg/mL solution
Caspase-3 Inhibitor (Ac-DEVD-CHO) (Part No. 90067): 50 μL of 100 μM (0.05 mg/mL) in DMSO
pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO
Stockage et stabilité
Store kit materials at -20°C up to their expiration date.
Special Precautions:
· After thawing reagents, use immediately or aliquot and freeze at -20°C for longer storage. Avoid repeated freeze/thaw cycles.
· The Caspase-3 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.
Special Precautions:
· After thawing reagents, use immediately or aliquot and freeze at -20°C for longer storage. Avoid repeated freeze/thaw cycles.
· The Caspase-3 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Code de la classe de stockage
10 - Combustible liquids
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Lingfang Yu et al.
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, 28(5), 879-890 (2015-07-26)
Lanthanide elements have been documented to possess various biologic effects, and their compounds have been studied intensely for their anti-cancer potential. However, the underlying mechanisms remain largely unknown. In the present study, we propose that the levels of proliferation and
Tibet Kacira et al.
Neurosurgery, 60(4), 674-679 (2007-04-07)
The purpose of this study is to explore whether or not the levels of caspase-3 (Casp3), neuron-specific enolase (NSE), and high-sensitivity C-reactive protein (hsCRP) were elevated in cerebrospinal fluid (CSF) and serum of patients after aneurysmal subarachnoid hemorrhage (SAH). This
Giuseppe M Campo et al.
Molecular and cellular biochemistry, 311(1-2), 167-177 (2008-01-29)
Previous studies suggest that Transforming growth factor-1beta (TGF-1beta) administration in human fibroblasts exposed to oxidative stress is able to modulate hyaluronan synthases (HASs). HAS modulation in turn increases high molecular weight (Hyaluronan) HA concentration. Nuclear factor kB (NF-kB) is a
M Uzan et al.
Acta neurochirurgica, 148(11), 1157-1164 (2006-09-12)
To determine whether sFas, caspase-3, proteins which propagate apoptosis, and bcl-2, a protein which inhibits apoptosis, would be increased in cerebrospinal fluid (CSF) in patients with severe traumatic brain injury (TBI) and to examine the correlation of sFas, caspase-3, and
Ana Faria et al.
Phytotherapy research : PTR, 24(12), 1862-1869 (2010-06-22)
The purpose of this study was to investigate the anticancer properties of an anthocyanin-pyruvic acid adduct extract, which is being developed aiming to be further applied in the food industry. An anthocyanin extract from blueberry (extract I) and an anthocyanin-pyruvic
Filtres actifs
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique
